en General General Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine Cytokines influence cell cycle events, which in some but not all instances can be associated with melanoma progression. Analysis of the G0/G 1 and S phase fractions of the cell cycle was used to assay the proliferative or inhibitory activity of cytokines against ten human melanoma cell lines, including pairs of cell lines derived from primary and metastatic tissue of individual patients. Cytokines and growth factors were generally capable of inducing either a proliferative or an inhibitory effect, depending upon the melanoma cell line tested. Distinct differences in responsiveness between the primary and metastatic partners of related pairs of melanoma cell lines was observed for IL-l β, IL-6, TNFα, TGFβ2, IFNα, IFNyand IGF-2. This data is complementary to previous works1.2 showing IL-6 and TGFβ2 to be inhibitory to.cell lines derived from early, but not advanced-stage melanomas. These alterations in cell cycle events were coincidental with a reduced expression of lCAM-1 and/or MHC antigen expression in response to some of these cytokines. Primary and metastatic melanoma, cytokine, cell cycle, proliferative and inhibitory effect, flow cytometry. 249 257 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-454&slc_lang=en&sid=1 AR ANDALIB 20031947532846004778 20031947532846004778 Yes FromEsfahan Medical School, Esfahan,I.R. Iran J LAWRY 20031947532846004779 20031947532846004779 No Sheffield University Medical School, UK BJ BURNS 20031947532846004780 20031947532846004780 No Sheffield University Medical School, UK AK MURRAY 20031947532846004781 20031947532846004781 No Sheffield University Medical School, UK M HERLYN 20031947532846004782 20031947532846004782 No Wistar Institute, Philadelphia, USA. RC REES 20031947532846004783 20031947532846004783 No Sheffield University Medical School, UK