Medical Journal of the Islamic Republic Of Iran

en Effect of three different sanitizing solutions on the contaminated bone: an experimental study in the rabbit Orthopedic Surgery Orthopedic Surgery Original Research Original Research Background: To determine the efficacy of three different antiseptic solutions (Control group (I), Antibiotic solution – Neomycin and polymyxin (II), Chlorhexidine 0.4% (III), and povidone – iodine 10% (IV)) in disinfecting contaminated bone fragments. Methods: Under sterile conditions, the femora of 12 rabbits were removed and cut into six millimeter pieces. A total of 200 bone specimens were obtained. All 200 specimens were dropped on the operating room floor for fifteen seconds and assigned to one of four experimental groups. Group I samples were cultured after immersion in normal saline solution (Control group). In other three groups, prior to culture the samples, they were washed with normal saline for ninety seconds and placed in an antibiotic solution (Neomycin & Polymyxin) (group II), Chlorhexidine 0.4% (group III), and povidone-iodine 10% (group IV) respectively. Results: In group I, 22 of 50 specimens had positive cultures. Of 50 specimens of group II and IV, positive cultures were found in 3 and 2 grafts respectively after 10 days whereas no positive cultures were detected in any samples of group III. Conclusion: Chlorhexidine 0.4% seems to be the best antiseptic solution for discontaminating the contaminated bone samples although it did not have any significant difference with povidone-iodine and other antibiotic solution. Sanitizing solutions, Contaminated bone. 114 117 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-315&slc_lang=en&sid=1 Hamidreza Yazdi dr.yazdi88@yahoo.com 20031947532846007450 20031947532846007450 No Tehran University of Medical Sciences, Tehran, Iran. Mehdi Ramezan Shirazi mehdi.shirazi@yahoo.co.uk. 20031947532846007451 20031947532846007451 Yes 15th Khordad Hospital, Gonabad University of Medical Sciences, Gonabad, Khorasan, Iran. Omid Momen Shouli momenshouli@yahoo.com 20031947532846007452 20031947532846007452 No Gilan University of Medical Sciences, Gilan, Iran. Farzad Yazdi farzad.yazdi@yahoo.com 20031947532846007453 20031947532846007453 No Shahrekord Azad University, Iran. Alireza Moslem alirezamoslem@gmail.com 20031947532846007454 20031947532846007454 No Gonabad University of Medical Sciences, Khorasan, Iran.