en Microbiology and Anatomy Microbiology and Anatomy Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) in the presence of 50% propanol- 1. Then equal suspensions of each conjugated serum were mixed to prepare V. cholera. Rectal swab samples from suspected choleric patients were inoculated in bile peptone broth for 5 hours at 37°C. One drop of each sample was mixed with one drop of VBCR and coagglutination was read at 2-3 minutes. The results were compared with corresponding results obtained from conventional culture methods. Specificity and sensitivity of coagglutination tests were found to be 98.03% and 95.1 %, respectively. Regarding the fact that rapid diagnosis of cholera is vital to save patients, our study reveals that coagglutination test, using bivalent mono-specific antisera, can be considered as a simple, rapid and reliable test to detect V. cholera-O 1 from stool samples of suspected patients. Vibrio cholera, Vibrio cholera bivalent mono-specific coagglutination reagent (VBCR), Classical culture method. 137 141 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-378&slc_lang=en&sid=1 SEYED ABBAS BAZARGAN 20031947532846006876 20031947532846006876 No From the Department of Microbiology, Medical School, Tarbiat Modarres University, Tehran BAHMAN TABARAIE 20031947532846006877 20031947532846006877 Yes the Department of Bacterial Vaccine and Antigen Production, P asteur Institute of Iran BAHRAM FATOLLAHZADEH 20031947532846006878 20031947532846006878 No From the Department of Microbiology, Medical School, Tarbiat Modarres University, Tehran NASRIN MOAZZAMI 20031947532846006879 20031947532846006879 No the Biotechnology Department, Iranian Research Organization/