Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1375
8
1
gregorian
1996
11
1
10
3
online
1
fulltext
en
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO CLOSTRIDIUM PERFRINGENS ENTEROTOXIN DERIVED FROM DIFFERENT ANIMAL SPECIES
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The enterotoxins (ETs) of Clostridium perfringens isolated from enterotoxemic
or diarrheic alpacas, pigs, calves, dogs, and horses were obtained from sporulated
cell extracts. The ETs from alpaca, pig, and calf isolates were chromatographed on
Sephadex G-lOO. Monoclonal antibodies (MAbs) against ETs derived from
alpaca, pig, and calf C. perfringens isolates were produced. The MAbs were used
in neutralization of cytotoxicity and immunoblot assays to study cross-reactivity
among all five ETs. Using neutralization of cytotoxicity technique, each MAb
exhibited neutralization against the cytopathic effect of all five ETs on Vero cells.
The neutralization ratio in this study was I ng ET: 50 ng MAb. On immunoblots,
the anti-alpaca ET and anti-pig ET cross-reacted with partially purified ETs from
pig and alpaca respectively. In addition, anti-alpaca ET and anti-pig ET crossreacted
with partially purified ETs from calf and horse, but not ET from dog
isolates. The anti-calf ET cross-reacted with alpaca, calf, dog, and pig but not with
ET from the horse isolate. The immunoblots also indicated that the MAbs
recognized aggregated ET subunits. Our data indicated that C. perfringens ETs
derived from different animal species are very similar.
C. perfringens; Enterotoxin; Monoclonal Antibody
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-431&slc_lang=en&sid=1
LAIA
GHARAVI NAEINI
20031947532846005352
20031947532846005352
Yes
From the National Research Center for Genetic Engineering and Biotechnology, Tehran, Islamic Republic of Iran
ROBERT P.
ELLIS
20031947532846005353
20031947532846005353
No
the Department of Microbiology, Colorado State University, Colorado, U.SA.