en Immunology Immunology Original Research Original Research Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleling the increase in accessory function, the cells decreased in the expression of markers typical of monocytes and macrophages (M 0), approaching the phenotype of lymphoid dendritic cells. We here define conditions for reproducibly generating these monocyte derived dendritic cells (m-DC) in various serum free media. This study also shows that acidic conditions prevent M0 development and facilitate m-DC differentiation even under serum conditions. 91 98 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-503&slc_lang=en&sid=1 ANNE CHRISTINE BRU-CAPDEVILLE 20031947532846005613 20031947532846005613 Yes From the Department of Immunology, University of Gouingen, West Germany HOSSAIN MOTTEIAN NAJAR 20031947532846005614 20031947532846005614 No JOHANN HINRICH PETERS 20031947532846005615 20031947532846005615 No