en Parasitology and Mycology Parasitology and Mycology Original Research Original Research <p> <strong> Abstract </strong><strong></strong></p><p> <strong> Objective: </strong>Shallots are important part of the diet for many people and there is </p><p>  long-held belief in their health enhancing properties. The aim of this study was to determine antifungal activity of shallot against reference fungal strains. </p><p> <strong> Methods: </strong>Alcoholic and aqueous extracts of shallot (Allium hirtifolium) were </p><p>  tested for in vitro antifungal activities against Aspergillus fumigatus, Aspergillus </p><p>  flavus, Aspergillus niger, Penicillium gryseogenum, Alternaria, Microsporum canis </p><p>  and Trichophyton mentagrophytes. The minimal inhibitory concentration (MIC) </p><p>  was determined using broth macrodilution method. The effects of shallot extracts </p><p>  were also compared with those of miconazole. </p><p> <strong> Results: </strong>Allium hirtifolium showed antifungal activity against all the </p><p>  fungi species tested with MIC values ranging from 0.058 to 0.8 mg/ml for alcoholic </p><p>  extract and 0.26 to 3.84 mg/ml for aqueous extract. The minimum fungicidal concentration (MFC) of alcoholic and aqueous extracts ranged from 0.1 to 12.8 mg/ml and 0.6 to 68.26mg/ml, respectively. </p><p> <strong> Conclusions: </strong>The results indicate that crude juice of shallot has antifungal activity </p><p>  and might be promising, at least, in treatment of fungal-associated diseases from </p><p>  mentioned fungi. </p><p>  </p> Allium hirtifolium, Miconazole, Aspergillus spp, Alternaria, Penicillium chrysogenum, Microsporum canis, Trichophyton mentagrophytes 17 22 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-94&slc_lang=en&sid=1 Roohollah Fateh 20031947532846001916 20031947532846001916 No Department of parasitology and Mycology, Iran University of Medical Sciences,Tehran, Iran Mohammad Javad Nasiri Kashani 20031947532846001917 20031947532846001917 No Department of Parasitology and Mycology, Iran University of Medical Sciences,Tehran, Iran. Manijheh Motevallian 20031947532846001918 20031947532846001918 No Razi Institue for Drug Research,Tehran, Iran. Mehraban Falahati mehrabanfalahati@yahoo.com 20031947532846001919 20031947532846001919 Yes Department of Parasitology and Mycology, Iran University of MedicalSciences,Tehran, Iran. Amir Yazdanparast 20031947532846001920 20031947532846001920 No Department of Parasitology and Mycology, Iran University of Medical Sciences,Tehran, Iran