en Health Health Original Research Original Research A field trial was canied outon60 volunteers selected in Yazd province, central Iran, with a vaccine containing killed promastigotes of L. major prepared by the Razi Institute, Hessarak. During these phase I studies which lasted for more than two years, we examined acceptable doses of the vaccine alone or mixed with BCG. The results so far indicate that 50 to 1000 µg of the vaccine alone can be well tolerated without major side-effects. The mixture of BCG with 400 µg of the vaccine produced pain and itching in five out of six volunteers, a self-limiting lymphadenopathy in one out of six, and fever in three out of six. Further trials are planned with reduced doses of BCG. The leishmania skin test became positive in a high proportion of the treated individuals after vaccination. Circulating antibodies were detected from two weeks to one month after vaccination. 211 215 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-560&slc_lang=en&sid=1 M MOHEBALI 20031947532846005976 20031947532846005976 Yes From the School of Public Health and Institute of Public Health research, Tehran university of Medical Science EH JAVADlAN 20031947532846005977 20031947532846005977 No From the School of Public Health and Institute of Public Health research, Tehran university of Medical Science R FESHARAKl 20031947532846005978 20031947532846005978 No Department of Protozoology and Entomology, Razi State Serum Vaccine Institute, Tehran, M MOHAMMADZADEH 20031947532846005979 20031947532846005979 No Amir al –Momenin Hospital, Shahid Sadooghi University of Medical Sciences, Yazd. Islamic Republic of Iran. A NADlM 20031947532846005980 20031947532846005980 No From the School of Public Health and Institute of Public Health research, Tehran university of Medical Science GH TAHYILDAR-BIDRUNI 20031947532846005981 20031947532846005981 No From the School of Public Health and Institute of Public Health research, Tehran university of Medical Science A MESDAGHINIA 20031947532846005982 20031947532846005982 No From the School of Public Health and Institute of Public Health research, Tehran university of Medical Science