Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1373
11
1
gregorian
1995
2
1
8
4
online
1
fulltext
en
DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
An enzyme-linked immunosorbent assay using a homologous combination of
antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin
(T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed.
This assay was utilized to measure testosterone in serum samples of male and female
subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossreacted
in less than 20% with 5α-dehydrotestosterone (5α DHT) . lnter- and
intraassay variations and all other validation factors such as recovery, test of
parallelism, etc. were well in the acceptable ranges. Comparison of testosterone
values of 32 plasma specimens obtained by solid phase ELISA method and
radioimmunoassay (RIA) showed a good correlation (0. 96).
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248
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-567&slc_lang=en&sid=1
GHR
ASADY KARAM
20031947532846006000
20031947532846006000
Yes
From the Department of Biochemistry. School of Medical Sciences.
MT
RASAEE
20031947532846006001
20031947532846006001
No