Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1372
5
1
gregorian
1993
8
1
7
2
online
1
fulltext
en
A SPECIFIC, SENSITIVE, AND ONE-STEP EXTRACTION RADIOIMMUNOASSAY OF TESTOSTERONE FOR CLINICAL DIAGNOSIS
Clinical Biochemistry
Clinical Biochemistry
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl
ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3-
O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study
exhibits minimal cross reactivity with the structurally related steroids specially 5
dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure
testosterone in female serum samples accurately with a high sensitivity, precision,
and specificity. The coefficent of variation (CY), standard deviation (SD) and
standard error of mean (SE) were all in acceptable ranges. Antibody-bound and free
steroids were separated by addition of dextran coated charcoal. The method was
applied to a set of clinical samples, the results of which are discussed in this
communication. The assay was compared with the available imported kits using 125
I as tracer. The correlation coefficient obtained is calcualted to be r= 0.96, showing
that the results obtained by these two methods are fully comparable and the assay may
be replaced with the similar preparations imported from abroad.
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108
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-644&slc_lang=en&sid=1
GH
R ASADIKARAM
20031947532846006193
20031947532846006193
Yes
From the Department of Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, I.R.lran
MJ
RASAEE
20031947532846006194
20031947532846006194
No
From the Department of Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, I.R.lran
S
ALE AGHA
20031947532846006195
20031947532846006195
No
the Quality.Control Department, Razi Research Institute, Karaj, I.R. Iran
GL
KUMARI
20031947532846006196
20031947532846006196
No
the Department of Reproductive Biomedicine, National Institute of health and Family Welfare,New Delhi, India
PN
RAO
20031947532846006197
20031947532846006197
No
the Department of Organic Chemistry. South West Foundation of Biomedical Research, San Antonio, Texas, USA.