en Clinical Biochemistry Clinical Biochemistry Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3- O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure testosterone in female serum samples accurately with a high sensitivity, precision, and specificity. The coefficent of variation (CY), standard deviation (SD) and standard error of mean (SE) were all in acceptable ranges. Antibody-bound and free steroids were separated by addition of dextran coated charcoal. The method was applied to a set of clinical samples, the results of which are discussed in this communication. The assay was compared with the available imported kits using 125 I as tracer. The correlation coefficient obtained is calcualted to be r= 0.96, showing that the results obtained by these two methods are fully comparable and the assay may be replaced with the similar preparations imported from abroad. 101 108 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-644&slc_lang=en&sid=1 GH R ASADIKARAM 20031947532846006193 20031947532846006193 Yes From the Department of Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, I.R.lran MJ RASAEE 20031947532846006194 20031947532846006194 No From the Department of Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, I.R.lran S ALE AGHA 20031947532846006195 20031947532846006195 No the Quality.Control Department, Razi Research Institute, Karaj, I.R. Iran GL KUMARI 20031947532846006196 20031947532846006196 No the Department of Reproductive Biomedicine, National Institute of health and Family Welfare,New Delhi, India PN RAO 20031947532846006197 20031947532846006197 No the Department of Organic Chemistry. South West Foundation of Biomedical Research, San Antonio, Texas, USA.