en Biological Sciences Biological Sciences Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine Alpha-amylase E.C.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as Pseudomonas and Asperigllus, as well as muscles and ovarian tubes.1.2 Alpha-amylase from Bacillus Subtilis #1024(A TCC 465)* was purified with a highest degree of purity in our laboratory (31.59 U/mg). The extracellular alpha-amylase was subjected to different purification techniques such as anionic and cationic exchange chromatography and preparative electrophoresis. The final fold of purification was equivalent to 38, which was higher than the previous reported values, polyacrylamide gel electrophoresis with sodium dodecyl sulphate (PAGESDS), Laemmeli method, gave a molecular weight of 82000 Daltons. Alpha-amylase, Bacterial enzymes, amylase electrophoresis 281 284 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-675&slc_lang=en&sid=1 FARIBA NABATCHlAN 20031947532846006262 20031947532846006262 Yes From the Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, P.O. Box '•1155-5399, Tehran, Islamic Republic of lran. BIJAN FARZAMI 20031947532846006263 20031947532846006263 No