Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1371
11
1
gregorian
1993
2
1
6
4
online
1
fulltext
en
PURIFICATIO N OF ALPHA-AMYLASE FROM BACILLUS SUBTILlS LINE # 1024 WITH ATCC 465
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Alpha-amylase E.C.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained
from salivary glands, pancreas and microorganisms such as Pseudomonas
and Asperigllus, as well as muscles and ovarian tubes.1.2 Alpha-amylase from
Bacillus Subtilis #1024(A TCC 465)* was purified with a highest degree of purity
in our laboratory (31.59 U/mg).
The extracellular alpha-amylase was subjected to different purification
techniques such as anionic and cationic exchange chromatography and preparative
electrophoresis.
The final fold of purification was equivalent to 38, which was higher than the
previous reported values, polyacrylamide gel electrophoresis with sodium dodecyl
sulphate (PAGESDS), Laemmeli method, gave a molecular weight of 82000
Daltons.
Alpha-amylase, Bacterial enzymes, amylase electrophoresis
281
284
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-675&slc_lang=en&sid=1
FARIBA
NABATCHlAN
20031947532846006262
20031947532846006262
Yes
From the Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, P.O. Box '•1155-5399, Tehran, Islamic Republic of lran.
BIJAN
FARZAMI
20031947532846006263
20031947532846006263
No