Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1371
11
1
gregorian
1993
2
1
6
4
online
1
fulltext
en
THE PRODUCTION OF MURINE MONOCLONAL ANTIBODIES (MAb) DIRECTED AGAINST HUMAN T- LYMPHOCYTE SUBSETS
Immunology
Immunology
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The production of murine monoclonal antibody (MAb) has not yet been reported
in Iran. The present work describes for the first time the generation of several
murine hybridoma clones secreting MAbs directed against human leukocyte
surface antigens. The secreted antibodies by hybridoma clones have been screened
on different lymphoid and non-lymphoid tissues. Results indicated that of seven
hybridomas, clones 3FII, 3C3 and IF2 showed a strong reactivity for T-cells
purified from thymus and tonsil tissues. Moreover, clones I D4 and 605 which
partially stained thymus tissues were found to be negative on purified B-cells and
monocytic cell line U937. The third group, 4ES and 4F4 hybridoma clones was
expressed weakly on purified T, B-cells and U937 cell line. Further work is needed
to determine the epitopes recognized by these MAbs and a comparison of the data
with those presented at the previous leukocyte antigens workshop.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-677&slc_lang=en&sid=1
ABBAS A.
GHADERI
20031947532846006267
20031947532846006267
Yes
From•the Department of Microbiology and Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz
Z
AMIRGHOFRAN
20031947532846006268
20031947532846006268
No
the Department of Immunology, Tarbiat Modarres University, Islamic Republic of Iran.