en Human Genetics Human Genetics Applicable Applicable <p> <strong> Abstract </strong></p><p> <strong> Background: </strong>Several studies suggested that some traits and polymorphisms in human genome such as G6PD deficiency and other genes have protective effects on susceptibility to malaria infection . </p><p> <strong> Methods: </strong>In present study we investigated the prevalence of TNF [1] -244GgA, TNF [1] - </p><p>  308 GgA,TNF [1] -238GgA, NOS2-954GgC, MBL54GgA, MBL 57GgA, MBL IVSI - </p><p>  5 GgA polymorphisms and G6PD variants (Mediterranean, Chatham, Cosenza, A - </p><p>  (202,376) in 315 subjects with G6PD deficiency and 10 malaria patient. All the 315 subjects were selected from five provinces (Fars, Khuzestan, Esfahan, Yazd and Kerman and screened by PCR-RFLP method . </p><p> <strong> Results: </strong>The NOS2-954GgA consisted GG(40.31%), GC(53.01%), and CC(6.66% ) </p><p>  where as TNF [1] -308 consisted GG(68.8%), AG(31.11% ) contents. The TNF [1] -244 </p><p>  showed GG(94.60%), AG(5.39%) genotypes and the TNF [1] -238 had GG(92.69% ), </p><p>  AG(6.66%), AA(0.63%) genotypes. The MBL54 polymorphism had GG(75.55%), AG (24.44%), AA(0.63%) genotypes. In MBL 57, had GG(95.23%), AG(4.76%), AA (0.63%) genotypes. The G6PD variants was indicated that Mediterranean mutation in Fars, Khuzestan, Esfahan, Yazd and Kerman provinces was 79.4%, 58%, 83/8%, 64% and 63% respectively and also, the Chatham mutation was 8.8%, 8% 4.5%,3.6% and 0% respectively. Analysis of other four mutations (Cosenza, Arures and A-202 and A-367 ) showed that none of them had those mutations . </p><p> <strong> Conclusion: </strong>Our results suggested that genotypes which causes protection against </p><p>  malaria or reduction of risk for celebral malaria and death has the maximum prevalence in samples taken from the five provinces, but in the kolmogorov-smiranov test results, only NOS2-954GgC supported the theory of relation between these polymorphisms and protection against malaria . </p><p>  </p> G6PD, Polymorphism, PCR-RFLP, TNF􀁄, MBL2, NOS2, Mediterranean, 57 66 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-102&slc_lang=en&sid=1 Mohammad Reza Noori-daloii nooridaloii@sina.tums.ac.ir 20031947532846001947 20031947532846001947 Yes Department of Medical Genetic, Faculty of Medicine, Tehran University of Medical Sciences, Pour Sina Mohammad Reza Alivand 20031947532846001948 20031947532846001948 No Departments of Medical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran Parisa Atef-Vahid 20031947532846001949 20031947532846001949 No Departments of Medical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. Feyzollah Hashemi-Gorji 20031947532846001950 20031947532846001950 No Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehrdad Hashemi 20031947532846001951 20031947532846001951 No Departments of Molecular Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.