Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1391
8
1
gregorian
2012
11
1
26
4
online
1
fulltext
en
Contaminated osteochondral plugs: effect of different sterilizing solutions: an experimental study in the rabbit
Orthopedic Surgery
Orthopedic Surgery
Original Research
Original Research
<font size="3" face="Times New Roman"> </font><font face="Calibri"><font size="3"><b>Background:</b> To determine the efficacy of different antiseptic solutions (Control group (I), Antibiotic solution (II), Chlorhexidine 0.4% (III), and povidone – iodine 10% (IV)) in sterilizing contaminated osteochondral plugs.<p></p></font></font><font size="3" face="Times New Roman"> </font><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 10pt" class="MsoNormal"><font face="Calibri"><font size="3"><b>Methods: </b>Under sterile conditions, the femoral head and condyles of 20 rabbits were removed and cut into equal osteochondral pieces. A total of 200 osteochondral specimens were obtained. All 200 specimens were dropped on the operating room floor for fifteen seconds and assigned to one of four experimental groups. Group I samples were cultured after washing with normal saline solution (Control group). In other three groups, prior to culturing process, samples were placed in an antibiotic solution after washing with normal saline (Neomycin & Polymyxin) (group II), Chlorhexidine 0.4% (group III), and povidone – iodine 10% (group IV), respectively.<p></p></font></font></p><font size="3" face="Times New Roman"> </font><font face="Calibri"><font size="3"><b>Results: </b>In group I, 25 of 50 specimens had positive cultures. Of 50 specimens of group II, III and IV, no positive cultures were found after 10 days.<p></p></font></font><font size="3" face="Times New Roman"> </font><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 10pt" class="MsoNormal"><font size="3"><font face="Calibri"><b>Conclusion:</b> all three agents including antibiotic solution, povidone-iodine 10% and chlorhexidine 0.4% seem effective in sterilizing the contaminated osteochondral samples. According to the literature, povidone-iodine has no negative effect on the cartilage metabolism and seems to be a proper choice of decontaminating solution for osteochondral plugs.To the best of the authors' knowledge, such a study on the contaminated osteochondral specimen has not been previously reported in the literature.<p></p></font></font></p><font size="3" face="Times New Roman"> </font>
Sterilizing, Contaminated, Osteochondral.
167
171
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-554&slc_lang=en&sid=1
Hamidreza
Yazdi
dr. yazdi88@yahoo.com
20031947532846009257
20031947532846009257
No
Shafa Yahyaian Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Mehdi
Ramezan Shirazi
mehdi.shirazi@yahoo.co.uk
20031947532846009258
20031947532846009258
Yes
Gonabad University of Medical Sciences, Gonabad, Iran.
Gholamreza
Shafiee
shafiee.gh@yahoo.com
20031947532846009259
20031947532846009259
No
Tehran University of Medical Sciences, Tehran, Iran.
Fereshteh
Shahcheraghi
m_shahcheraghi@yahoo.com
20031947532846009260
20031947532846009260
No
Iran Pasteur institute of Microbiology, Tehran, Iran.