en Pathology Pathology Original Research Original Research <p> <strong> Abstract </strong></p><p> <strong> Background: </strong>Squamous cell carcinoma (SCC) constitutes the main oral malignancy . </p><p>  Parallel to better understanding of molecular and genetic patterns of tumor behavior, more precise correlation of tumor markers such as Epidermal Growth Factor (EGF) and Laminin-5 are sought to estimate macroscopic and microscopic tumor status . </p><p> <strong> Methods: </strong>We conducted a cross-sectional study collecting oral SCC samples during </p><p>  2006-2007 from Pathology Department of Shahid Beheshti Dental School. Mmunohistochemical staining with antibodies against EGFR and laminin-5 along with staining degree were reported by two experienced pathologist including degree of staining (low, medium , high), and pathological grading and clinical staging obtained from medical records . </p><p> <strong> Results: </strong>Forty two patients' paraffin blocks of SCC examined with mean age 58( 18.72 ) yrs ranged between 21-88, female to male ratio of 1.33:1 was observed. The study analyses revealed a significant correlation between the expression of laminin-5 with tumor stage and grade (P< 0.001 r=0.547 and r=0.545 respectively), yet no significant correlation between expression of EGFR and tumor stage or grade (P=0.894 r=-0.018 and P=0.543 r=0.86 respectively). Considering high degree of staining and stage IV sensitivity, specifity , positive predictive value (PPV) and negative predictive value (NPV) of 44%, 54%, 44% and 78% calculated for ERGF and 55%, 78%, 58% and 86% for laminin-5 respectively . Considering high degree of staining and grade 3 sensitivity, specifity, positive predictive value (PPV) and negative predictive value (NPV) of 57%, 57%,17% and 86% calculated for EGFR and 85%, %82, 50% and 96% for Laminin-5 respectively . </p><p> <strong> Conclusion: </strong>We concluded that laminin-5 has a better prediction for developing higher tumor stage or grade but further research needed for identifying the precise role of EGFR . </p> Epidermal growth factor, laminin-5, SCC, stage, grade, negative predictive value 146 150 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-118&slc_lang=en&sid=1 Shima Nafarzadeh shima_nafar2004@yahoo.com. 2003194753284600871 2003194753284600871 No Department of Maxillofacial Pathology, Dentistry school, Babol University of Medical Sciences, Babol, Iran Mohammad Moshref moshrefdds@yahoo.com 20031947532846001039 20031947532846001039 No Department of Maxillofacial Pathology, Dentistry school, Shahid Beheshti University of Medical Sciences, Tehran, Iran Fatemeh Mashhadi Abbass f_mashhadi_a@yahoo.com 20031947532846001040 20031947532846001040 No Department of Maxillofacial Pathology, Dental school, Shahid Beheshti University of medical sciences, Tehran, Iran Zohreh Mohammad Taheri ddsdr22@yahoo.com 20031947532846001041 20031947532846001041 No Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Arash Poorsattar Bejeh Mir a.poorsattar@mubabol.ac.ir 20031947532846001042 20031947532846001042 Yes Iran University of Medical Sciences, Tehran, Iran. Dentistry student, Central student research committee Dentistry school, Babol University of Medical Sciences, Babol, Iran