en Exercise Physiology Exercise Physiology Original Research Original Research <p> <strong> Background: </strong>Heavy exercise cause muscle damage associated with production of inflammatory agents. The purpose of present study was to determine the effect of acute and 14-day Coenzyme Q10 supplementation on inflammatory, blood lactate and muscle damage in male middle-distance runners. </p><p> <strong> Methods </strong>: Eighteen male middle-distance runners in a randomized and quasi experimental study were allocated into two equal groups: supplement group (n=9, Coenzyme Q10: 5mg/kg/day) and placebo group (n=9, Dextrose: 5mg/kg/day). After acute (1day) and 14-day supplementation, all subjects were participated in a training like running (competitive 3000 meters). Blood samples were obtained in the four phases: one hour before and 18-24 hours after two running protocols. Lactate, serum interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), C-reactive protein (CRP) and creatine kinase (CK) were analyzed. Repeated ANOVA and Bonferunias a post hoc tests were used to determine the changes in four stages. Differences between groups were determined by t-test. </p><p> <strong> Results </strong>: The results showed that acute and short-term Coenzyme Q10 supplementation had not significant effect on basal parameters. The acute coenzyme Q10 supplementation attenuated only the exercise-induced increase in response of the plasma CRP. The short-term (14-day) coenzyme Q10 supplementation attenuated the exercise-induced increase in response of the lactate, serum interleukin-6, tumor necrosis factor-alpha, and CRP in male middle-distance runners. However, the acute and short-term coenzyme Q10 supplementation had not any significant effect on the exercise-induced increase response of total serum creatine kinase. </p><p> <strong> Conclusion </strong>: Based on the present results, it can be concluded that the 14-day coenzyme Q10 supplementation (5mg.kg-1.day-1) is more effective than the acute supplementation to overcome the exercise-induced adverse responses in some oxidative, inflammatory and biochemical parameters. Therefore, short-term coenzyme Q10 supplementation is recommended to reduce exercise-induced adverse consequences. </p> Runners, Coenzyme Q10, Inflammatory, Lactate, Muscle damage. 345 352 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-833&slc_lang=en&sid=1 Mostafa Armanfar Mostafaarmanfar@yahoo.com or marmanfar@tabrizu.ac.ir 200319475328460016449 200319475328460016449 Yes Exercise Physiology Department, University of Tabriz, Tabriz, Iran. Afshar Jafari ajafari.@tabrizu.ac.ir 200319475328460016450 200319475328460016450 No Department of Physical Education, University of Tabriz, Tabriz, Iran. Gholam Reza Dehghan dehghan2001.d@yahoo.com 200319475328460016451 200319475328460016451 No Department of Biochemistry, University of Tabriz, Tabriz, Iran. Leila Abdizadeh L.Abdizadeh @yahoo.com 200319475328460016452 200319475328460016452 No Department of Sport Physiology, Faculty of physical education and sport sciences, Islamic Azad University, Karaj, Iran.