Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1393
10
1
gregorian
2015
1
1
29
1
online
1
fulltext
en
Reduction in ischemic brain injury following the administration of pentoxifylline after transient global ischemia/ reperfusion in a rat model
Cellular & Molecular Research
Cellular & Molecular Research
Original Research
Original Research
<p> <strong> Background: </strong>It is well known that the hippocampus, the CA1 Pyramidal cells in particular, is selectively vulnerable during global cerebral ischemia. Recently, it is observed that pentoxifylline has a neuroprotective effect. This study explored the pharmacological relationship between ischemia-induced cell death of the hippocampus and the efficacy of a vasodilator agent (pentoxifylline) in the prevention of delayed neuronal death. </p><p> <strong> Methods </strong>: This experimental study was performed on 4 groups: control, ischemia, experimental (200mg/kg pentoxifylline injection one hour prior to and one hour following ischemia) and vehicle (normal saline). Transient global ischemia was induced by bilateral common carotid arteries occlusion. To investigate the apoptotic bodies and caspase-3 activities as a central role in the execution phase of apoptosis, the brains were prepared for the TUNEL technique. </p><p> <strong> Results </strong>: Pentoxifylline administration limited apoptosis and caspase-3 activities in rats’ hippocampi. Our data showed no significant difference between the number of apoptotic bodies in the CA1 region of the hippocampus in the control and pentoxifylline -treated groups (p= 0.994). The results of one- way ANOVA revealed that that ischemia significantly increased caspase-3 levels in the hippocampus (p<0.05) however, the level of caspase-3 in pentoxifylline -treated rats was less than the ischemic group. </p><p> <strong> Conclusion </strong>: These results suggest that the neuroprotective effect of pentoxifylline (200mg/kg) may be accompanied by a reduction in ischemic damage within the CA1 region of the hippocampus in rats subjected to transient global cerebral ischemia. </p>
Cerebral ischemia, Reperfusion, Pentoxifylline, Hippocampus.
279
287
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-835&slc_lang=en&sid=1
Zahra Nadia
Sharifi
nadiasharifi@yahoo.com
200319475328460016459
200319475328460016459
No
Department of Anatomy, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.
Shabnam
Movassaghi
sm_movassaghi@yahoo.com
200319475328460016460
200319475328460016460
No
Department of Anatomy, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.
Farzaneh
Mohamadzadeh
farzaneh.mohammadzadeh@yahoo.com
200319475328460016461
200319475328460016461
No
Departmentof Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Sara
Soleimani Asl
S.soleimaniasl@umsha.ac.ir
200319475328460016462
200319475328460016462
No
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Bagher
Pourheydar
bagher.pourheydar@gmail.com
200319475328460016463
200319475328460016463
No
Neurophysiology Research Center, Department of Anatomy, Urmia University of Medical Sciences, Urmia, Iran.
Mehdi
Mehdizadeh
mehdizadeh.m@iums.ac.ir
200319475328460016464
200319475328460016464
Yes
Cellular and Molecular Research Center, Faculty of Advanced Technologies in Medicine, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran