en Parasitology and Mycology Parasitology and Mycology Original Research Original Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an<br> important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the<br> cyst, has been investigated.<br> <strong>Methods: </strong>The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst fluid (CF) and protoscolex<br> (PS) antigens of E. Granulosus using ELISA and western immunoblotting tests. Thin-layer chromatography and &szlig;-elimination<br> were used for glycan purification.<br> <strong>Results:</strong> Both hydatid cyst and normal human sera reacted with hydatid cyst fluid, protoscolices, laminated layer, glycoprotein and<br> glycolipid antigens. The most antigen-antibody reaction was related to CF and PS antigens, and LL antigens had the minimal reaction<br> with the sera. Thin layer chromatography (TLC) of the antigens showed presence of many glycan bands in the laminated layer.<br> <strong>Conclusion:</strong> The parasite may elaborate different glycan antigens in LL to evade host immune response.</p> Echinococcus granulosus, Hydatid cyst, Laminated Layer, Thin-layer chromatography, Enzyme Linked Immunosorbent Assay, ß-elimination 66 70 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-2486-1&slc_lang=en&sid=1 Raheleh Rafiei Sefiddashti 200319475328460031204 200319475328460031204 No Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran. Seyedeh Maryam Sharafi 200319475328460031205 200319475328460031205 No Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Soltan Ahmad Ebrahimi 200319475328460031206 200319475328460031206 No Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Lame Akhlaghi 200319475328460031207 200319475328460031207 No Department of Medical Parasitology and Mycology, Faculty of Medicine, Iran university of Medical Sciences, Tehran, Iran. Ali Moosavi 200319475328460031208 200319475328460031208 No Iran University of Medical Sciences, Tehran, Iran. Abbasali Eskandarian 200319475328460031209 200319475328460031209 No Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran. Alireza Hejrati 200319475328460031210 200319475328460031210 No Iran University of Medical Sciences, Tehran, Iran. Hossein Yousofi Darani 200319475328460031211 200319475328460031211 Yes Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.