en Medical Mycology and Biotechnology Medical Mycology and Biotechnology Original Research Original Research <p> <strong>ABSTRACT</strong></p><p> Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years. Cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (CNS) in immunocompetent as well as immunocompromised patients. We report the development of a semi-nested- PCR-based assay for the detection of C. neoformans in less than 100 yeast cells per ml of cerebrospinal fluid (CSF).</p> Cryptococcus neoformans, PCR, Semi-nested PCR, CSF 62 65 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-209&slc_lang=en&sid=1 SHAHINDOKHT BASSIRI JAHROMI 20031947532846002316 20031947532846002316 Yes MANSOOR ABACHI 20031947532846002317 20031947532846002317 No ALI ASGHAR KHAKSAR 20031947532846002318 20031947532846002318 No