Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1394
10
1
gregorian
2016
1
1
30
1
online
1
fulltext
en
Radioprotective effects of selenium and vitamin-E against 6MV X-rays in human blood lymphocytes by micronucleus assay
Radiography
Radiography
Original Research
Original Research
<p dir="ltr" style="text-align: justify"><strong>Background: </strong>Critical macromolecules of cells such as DNA are in exposure to damage of free radicals that induced from the interaction of ionizing radiation with biological systems. Selenium and vitamin-E are natural compounds that have been shown to be a direct free radical scavenger. The aim of this study was to investigate the radioprotective effect of selenium and vitamin-E separately and synergistically against genotoxicity induced by 6MV x-rays irradiation in blood lymphocytes.</p>
<p dir="ltr" style="text-align: justify"><strong> Methods</strong>: Fifteen volunteers were divided into three groups include A, B and C. These groups were given selenium (800IU), vitamin-E (100mg) and selenium (400IU) + vitamin-E (50mg), respectively. Peripheral blood samples were collected from each group before (0hr) and 1, 2 and 3hr after selenium and vitamin-E administration (separately and synergistically). Then the blood samples were irradiated to 200cGy of 6MV x-rays. After that lymphocyte samples were cultured with mitogenic stimulation to determine the chromosomal aberrations with micronucleus assay in cytokinesis-blocked binucleated cells.</p>
<p dir="ltr" style="text-align: justify"><strong> Results</strong>: The lymphocytes in the blood samples collected at one hr after ingestion selenium and vitamin-E, exposed in vitro to x-rays exhibited a significant decrease in the incidence of micronuclei, compared with control group at 0hr. The maximum protection and decrease in frequency of micronuclei (50%) were observed at one hr after administration of selenium and vitamin-E synergistically.</p>
<p dir="ltr" style="text-align: justify"><strong> Conclusion</strong>: The data suggest that ingestion of selenium and vitamin-E as a radioprotector substance before exposures may reduce genetic damage caused by x-rays irradiation.</p>
6MV, X-rays, Selenium, Vitamin-E, Lymphocyte, Micronucli.
469
475
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-999&slc_lang=en&sid=1
Aram
Rostami
rostamy_1969@yahoo.com
200319475328460022384
200319475328460022384
No
Department of Medical Physics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Seyed Akbar
Moosavi
seyedmoos@gmail.com
200319475328460022385
200319475328460022385
No
Department of Lab Sciences, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Vahid
Changizi
changizi@sina.tums.ac.ir
200319475328460022386
200319475328460022386
Yes
Department of Technology of Radiology and Radiotherapy, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ali
Abbasian Ardakani
a.ardekani@live.com
200319475328460022387
200319475328460022387
No
Department of Medical Physics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.