Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1384
2
1
gregorian
2005
5
1
19
1
online
1
fulltext
en
ADENOSINE DEAMINASE ACTIVITY IN ESTROGEN RECEPTOR POSITIVE AND NEGATIVE HUMAN BREAST CANCER CELL LINES
Clinical Biochemistry
Clinical Biochemistry
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
<p> <strong>ABSTRACT</strong></p><p> <strong>Background:</strong> The aims of this study were to assay the activity of adenosine deaminase (ADA) in estrogen receptor positive (MCF-7) and negative (MDA-MB468) breast cancer cell lines.</p><p> <strong>Methods:</strong> MDA-MB468 and MCF-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-LM diethylstilbestrol (DES), complete medium in the presence and absence of 111M tamoxifen for 20 hr. Adenosine deaminase activity was determined using the colorimetric method described by Guisti and Galanti.</p><p> <strong>Results:</strong> It was found that the activity of enzyme in estrogen receptor positive (ER+) cell line (MCF-7) was significantly higher than that of estrogen receptor negative breast cancer cell line (MDA-MB468). ADA activity in MCF-7 cells cultured in the presence of tamoxifen or charcoal-striped serum was significantly lower than that of control. Furthermore addition of diethylstilbestrol (DES) to the striped serum increased the value of ADA activity to that of control. Unlike MCF-7 cells, the activity of ADA in MDA-MB468 cells remained unchanged upon treatment with tamoxifen or striped serum.</p><p> <strong>Conclusion:</strong> These findings suggest estrogen responsiveness of ADA expression in MCF-7 cells.</p>
Adenosine deaminase, breast cancer cell lines, estrogen receptor.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-261&slc_lang=en&sid=1
MOHAMMAD
HASHEMI
mhdhash@yahoo.com
20031947532846006682
20031947532846006682
Yes
Dept. of Clinical Biochemistry, Zahedan Medical University, Zahedan , Iran .
FATEMEH
KARAMI TEHRANI
20031947532846006683
20031947532846006683
No
Cancer Research Lab, Dept. of Clinical Biochemistry, School of Medical Sciences, Tarbiat ModarresUniversity, Tehran, Iran.
SAEID
GHAVAMI
20031947532846006684
20031947532846006684
No
Dept. of Clinical Biochemistry, School of Medicine, Zahedan Medical University, Zahedan, Iran,
MAJID
SIRATI SABET
20031947532846006685
20031947532846006685
No
Dept. of Clinical Biochemistry, School of Medicine, Qazvin MedicalUniversity, Qazvin, Iran.