Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1395
10
1
gregorian
2017
1
1
31
1
online
1
fulltext
en
Prevalence of proline racemase/ hydroxyproline epimerase gene in human brucella isolates in Iran
Medical Bacteriology
Original Research
Original Research
<p><strong>Background: </strong>Human brucellosis is a zoonotic disease caused by Brucella melitensis, Brucella abortus, and Brucella suis. Brucella causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important Brucella virulence determinant, PrpA, which can modulate immune response, was determined in human isolates.<br>
<strong>Methods:</strong> Polymerase chain reaction (PCR) assay was standardized and applied to 37 isolates obtained from patient’s specimens. Primers for prpA gene were designed and evaluated using bioinformatic tools. DNA sequencing was performed for further verification.<br>
<strong>Results: </strong>In the 37 Brucella isolates (31 Brucella melitensis and 6 Brucella abortus), 32 (86.4%) carried prpA gene.<br>
<strong>Conclusion: </strong>Presence of prpA gene in most isolates indicates the high prevalence of this gene among Iranian isolates and emphasizes its role in pathogenicity of this organism.<br>
<br>
</p>
Brucella species, Brucellosis, Proline Racemase, PrpA
332
335
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3259-1&slc_lang=en&sid=1
Iman
Hashemifar
Hashemifar.iman@gmail.com
200319475328460033997
200319475328460033997
No
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Faramarz
Masjedian Jazi
fmasjedian@yahoo.com
200319475328460033998
200319475328460033998
No
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abbas
Yadegar
babak_y1983@yahoo.com
200319475328460033999
200319475328460033999
No
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastro-enterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nour
Amirmozafari
amirmozafari@yahoo.com
200319475328460034000
200319475328460034000
Yes
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.