en Virology Virology Original Research Original Research <p><strong>Background: </strong>Human parvovirus B19 was known as one of the possible cause of mild respiratory tract diseases in previous studies. However, there are some reports of acute obstructive respiratory disease and severe pneumonia. The purpose of current study was to assess the prevalence and clinical features of parvovirus B19 in respiratory infection.<br> <strong>&nbsp;&nbsp; Methods:</strong> This study was conducted on 156 patients diagnosed with respiratory infection at the Iran University of Medical Sciences-affiliated hospitals. After extraction of viral DNA from swab samples, detection of parvovirus B19 was performed by real-time PCR assay.<br> <strong>&nbsp;&nbsp; Results: </strong>In 156 patient&rsquo;s samples, parvovirus B19 was found in 8 (5.1 %) cases including 5 males (5.9%) and 3 females (4.1%). The most common clinical symptoms were wheezing (100%), tachypnea (100%), fever (75%) and rhinorrhea/pharyngitis (75%).<br> <strong>&nbsp;&nbsp; Conclusion: </strong>This is the first attempt to assess the prevalence of parvovirus B19 infection in Iranian patients with respiratory infection. The low frequency of parvovirus B19 detected in our study does not support the role of this virus in the development of respiratory infection. However, further studies are needed to better evaluate the etiological role of parvovirus B19 in respiratory infection.<br> &nbsp;<br> &nbsp;</p> Parvovirus B19, Real-time PCR, Respiratory infection, Iran 220 223 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-2447-3&slc_lang=en&sid=1 Ahmad Tavakoli ahmad.tavakoli66@gmail.com 200319475328460037525 200319475328460037525 No Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Seyed Hamidreza Monavari hrmonavari@yahoo.com 200319475328460037526 200319475328460037526 Yes Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Hamidreza Mollaei tavakoli@email.com 200319475328460037527 200319475328460037527 No Department of Microbiology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Farah Bokharaei-Salim bokharaeifarah@gmail.com 200319475328460037528 200319475328460037528 No Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Maryam Esghaei maryam.esghaei@gmail.com 200319475328460037529 200319475328460037529 No Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Hossein Keyvani keyvanlab@yahoo.com 200319475328460037530 200319475328460037530 No Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Hadi Ghaffari ghaffari1364@yahoo.com 200319475328460037531 200319475328460037531 No Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.