Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1396
11
1
gregorian
2018
2
1
32
1
online
1
fulltext
en
The relationship between embb306 and embb406 mutations and ethambutol resistant in Mycobacterium tuberculosis isolated from patiens in west of Iran
Medical Bacteriology
Medical Bacteriology
Brief Communication
Brief Communication
<p><strong> Background: </strong>Mutations in embB gene have been reported in ethambutol (EMB) resistance Mycobacterium tuberculosis (M. tuberculosis) isolates. The aim of this study was survey on embB 306 and 406 EMB resistant M. tuberculosis isolated from patients in West of Iran (2014-2015).<br>
<strong> Methods</strong>: Fifty strains of M. tuberculosis from patients with pulmonary tuberculosis (TB) were considered. Drug susceptibility using proportional method was performed. Polymerase chain reaction (PCR) -DNA sequencing was applied for mutation in embB 306 and 406 codon detection. Data were analyzed in SPSS 16 software using descriptive statistics and Fisher's exact test (p<0.05).<br>
<strong> Results: </strong>In this study 7 (14%) M. tuberculosis isolates were resistant to EMB. 6 (85.71%) and 1 (14.28%) resistant isolates had embB 306 and 304 codon mutations, respectively. Between embB306 mutations and resistance to EMB and MDR isolates had a significant relationship (p<0.001).<br>
<strong> Conclusion</strong>: The data indicated that embB 306 mutations have effect on EMB resistant. Detection of EMB resistant and these mutations prominent for antibiotic prescription.<br>
</p>
embB gene, Mutations, Ethambutol Resistance, Mycobacterium tuberculosis
686
689
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3342-1&slc_lang=en&sid=1
Bahman
Mohammadi
hamidmoqhtader@yahoo.com
200319475328460042161
200319475328460042161
No
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
Parviz
Mohajeri
microbiol_sci2013@yahoo.com
200319475328460042162
200319475328460042162
No
Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Samaneh
Rouhi
roohi.samaneh@yahoo.com
200319475328460042163
200319475328460042163
No
Student Research Committee & Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
Rashid
Ramazanzadeh
microbiol_sci@yahoo.com
200319475328460042164
200319475328460042164
Yes
Cellular and Molecular Research Center, Research Institute for Health Development & Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran