en Nutrition Nutrition Original Research Original Research <strong>Background: </strong>Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-&kappa;B and AP-1 transcription factors.<br> <strong>&nbsp;&nbsp; Methods:</strong> HepG2 cells were settled in a serum- free medium with high concentrations of glucose (30 mM) and insulin (1 &micro;M) overnight and were then incubated with RSV (5, 10, and 20 &micro;M) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression.<br> <strong>&nbsp;&nbsp; Results:</strong> RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose- dependently after 24 and 48 hours (p<0.05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0.05). Our results indicated that RSV may reduce NF-&kappa;B and AP-1 activity via RelA and c-Jun gene regulation.<br> <strong>&nbsp;&nbsp; Conclusion: </strong>The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC). AP-1, c-Jun, Hyperglycemia, Hyperinsulinemia, Inflammation, Resveratrol, NF kappa B, MAPK 51 56 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3693-1&slc_lang=en&sid=1 Zinat Mohammadpour znt.mohamadpur@yahoo.com 200319475328460036650 200319475328460036650 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran & Liver Transplantation Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran. Fatemehsadat Amiri sadafamiri.2011@yahoo.com 200319475328460036651 200319475328460036651 No Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Ali Akbar Saboor-Yaraghi saboor_a@yahoo.com 200319475328460036652 200319475328460036652 No Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fariba Koohdani fkoohdan@tums.ac.ir 200319475328460036653 200319475328460036653 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Marjan Norouzzadeh marjan_dietition@yahoo.com 200319475328460036654 200319475328460036654 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Loghman Sharifi loghmansharifi@yahoo.com 200319475328460036655 200319475328460036655 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Monireh Sadat Seyyed Salehi salehy.monir@yahoo.com 200319475328460036656 200319475328460036656 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Amirpasha Ebrahimi ebrahimi.amirpasha@yahoo.com 200319475328460036657 200319475328460036657 No Liver Transplantation Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran. Maryam Mahmoudi m-mahmoudi@sina.tums.ac.ir 200319475328460036658 200319475328460036658 Yes Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran & Pediatric Gastroenterology and Hepatology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran & Dietetics and Nutrition Experts Team (DiNET), Universal Scientific Education and Research Network (USERN), Tehran, Iran.