Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1396
11
1
gregorian
2018
2
1
32
1
online
1
fulltext
en
Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation
Nutrition
Nutrition
Original Research
Original Research
<strong>Background: </strong>Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-κB and AP-1 transcription factors.<br>
<strong> Methods:</strong> HepG2 cells were settled in a serum- free medium with high concentrations of glucose (30 mM) and insulin (1 µM) overnight and were then incubated with RSV (5, 10, and 20 µM) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression.<br>
<strong> Results:</strong> RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose- dependently after 24 and 48 hours (p<0.05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0.05). Our results indicated that RSV may reduce NF-κB and AP-1 activity via RelA and c-Jun gene regulation.<br>
<strong> Conclusion: </strong>The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC).
AP-1, c-Jun, Hyperglycemia, Hyperinsulinemia, Inflammation, Resveratrol, NF kappa B, MAPK
51
56
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3693-1&slc_lang=en&sid=1
Zinat
Mohammadpour
znt.mohamadpur@yahoo.com
200319475328460036650
200319475328460036650
No
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran & Liver Transplantation Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Fatemehsadat
Amiri
sadafamiri.2011@yahoo.com
200319475328460036651
200319475328460036651
No
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Ali Akbar
Saboor-Yaraghi
saboor_a@yahoo.com
200319475328460036652
200319475328460036652
No
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Fariba
Koohdani
fkoohdan@tums.ac.ir
200319475328460036653
200319475328460036653
No
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
Marjan
Norouzzadeh
marjan_dietition@yahoo.com
200319475328460036654
200319475328460036654
No
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
Loghman
Sharifi
loghmansharifi@yahoo.com
200319475328460036655
200319475328460036655
No
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
Monireh Sadat
Seyyed Salehi
salehy.monir@yahoo.com
200319475328460036656
200319475328460036656
No
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
Amirpasha
Ebrahimi
ebrahimi.amirpasha@yahoo.com
200319475328460036657
200319475328460036657
No
Liver Transplantation Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Maryam
Mahmoudi
m-mahmoudi@sina.tums.ac.ir
200319475328460036658
200319475328460036658
Yes
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran & Pediatric Gastroenterology and Hepatology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran & Dietetics and Nutrition Experts Team (DiNET), Universal Scientific Education and Research Network (USERN), Tehran, Iran.