Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1398
11
1
gregorian
2020
2
1
34
1
online
1
fulltext
en
Analysis of FGFR3 and HRAS genes in patients with bladder cancer
Medical Genetics
Medical Genetics
Original Research
Original Research
<strong>Background: </strong>Bladder cancer is the most frequent malignancy that affects the urinary tract. Studies have shown different types of FGFR3 and HRAS genes mutations in human bladder cancer, with a comprehensive range of mutation number in various populations. This study aimed to determine the specific point mutations of these 2 genes among Iranian patients with bladder cancer.<br>
<strong>Methods:</strong> In this study, 100 specimens of patients with transitional cell carcinoma were analyzed. All samples were examined for FGFR3 and HRAS mutations using PCR and direct DNA sequencing methods.<br>
<strong>Results:</strong> A total of 9 pathogenic mutations and 9 polymorphisms were found in 2 exons (7 and 15) of the FGFR3 genes in patients with bladder cancer (S249Y, I633I, L645L, D646E, Y647*, D628V, P250T, Q263H, Y305H). However, no mutation was found in exon 10 of FGFR3 and exon 1 of HRAS genes.<br>
<strong>Conclusion:</strong> In this study, 5 mutations were found in FGFR3 gene that have not been detected previously. There was no mutation in exon 10 of FGFR3 and exon1 of HRAS. The results of this study confirmed the association of ethnic-genetic factors in the occurrence of bladder cancer, so that these variables may not be present in all ethnic groups.<br>
TCC, FGFR3, HRAS, FFPE, Touch down PCR
741
746
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3949-1&slc_lang=en&sid=1
Ameneh
Homami
amena.hilli@yahoo.com
200319475328460064307
200319475328460064307
No
Department of Medical Genetics and Molecular Biology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Zohreh
Ataei Kachoei
abc1349@yahoo.com
200319475328460064308
200319475328460064308
No
Department of Medical Genetics and Molecular Biology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Mojgan
Asgarie
mojgan_asgari@yahoo.com
200319475328460064309
200319475328460064309
No
Department of Medical Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Farideh
Ghazi
Ghazi.f@iums.ac.ir
200319475328460064310
200319475328460064310
Yes
Department of Medical Genetics and Molecular Biology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran