Medical Journal of the Islamic Republic Of Iran

en EFFECTS OF UBIQUINOL-1 0 AND f3-CAROTENE ON THE IN VITRO SUSCEPTIBILITY OF LOW-DENSITY LIPOPROTEIN TO COPPER-INDUCED OXIDATION Special Special Original Research Original Research ABSTRACT<o:p /> Background: Dietary antioxidant intake has been reported to be inversely associated with coronary miery disease. To clarify the possible role oflipophilic antioxidants in the prevention of atherosclerosis, we investigated the effects ofubiquinol-1 0 and ~-carotene on the susceptibility oflow-density lipoprotein(LD L) to oxidative modification.<o:p /> Methods: In this study, first "ubiquinol-1 0 and ~-carotene" were added to plasma and incubated for 3hr at 37°C. Then, the LDL fraction was separated by ultracentrifugation. The oxidizability ofLDL was estimated by measuring conjugated diene (CD), lipid peroxides and thiobarbituric acid-reactive substances (TBARS) after cupric sulfate solution was added.<o:p /> Results: We showed that ubiquinone-! 0 and ~-carotene significantly (p<O.Ol by ANOVA) and dose-dependently prolonged the lag time before initiation of oxidation reaction. Also, these two compounds suppressed the formation oflipid peroxides and TBARS more markedly than others. The ability of them to prolong lag time and suppression oflipid peroxides and TBARS formation resulted to be in the following order: ubiquinol-1 0> ~-carotene.<o:p /> Conclusion: LDL exposed to the lipophilic antioxidants in vitro reduced oxidizability. These findings suggest that ubiquinol-1 0 and ~-carotene have a role in ameliorating atherosclerosis.<o:p /> LDL, Oxidation, Atherosclerosis, Ubiquinol -1 0,β -Carotene . 169 174 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-308&slc_lang=en&sid=1 MOHAMMAD-REZA SAFARI safa ri (i:i ·umsha .ac.ir 20031947532846003040 20031947532846003040 Yes Department of Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciencesand Health Services, Hamadan, Iran. MOHSEN REZAEI 20031947532846003041 20031947532846003041 No Department of Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciencesand Health Services, Hamadan, Iran. HESHMATOLLAH TAHERKHANI 20031947532846003042 20031947532846003042 No Department of Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciencesand Health Services, Hamadan, Iran.