en Parasitology and Mycology Parasitology and Mycology Original Research Original Research <strong>&nbsp;&nbsp;</strong><strong>&nbsp; Background: </strong>Toxoplasma gondii (T. gondii) is the most common parasite that can lead to a disease called toxoplasmosis. In this study, serological and molecular complementary tests have been conducted to detect or diagnose this parasite.<br> &nbsp;&nbsp; <strong>Methods:</strong> A total of 71 patients with clinical symptoms of ocular toxoplasmosis and 20 patients with other ocular infections were evaluated. Serum and buffy coat samples were collected and tested using enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (nPCR) assessments. Superficial T. gondii B1 gene was evaluated in PCR. The ocular toxoplasmosis patients were followed-up 2 weeks after the first sampling and 4 weeks following the first laboratory testing. The main outcome measures were the efficiency of the diagnostic procedure and positive and negative predictive values (PPV and NPV).<br> &nbsp;&nbsp; <strong>Results: </strong>Overall, of the samples, 69% were PCR+, IgG+, and IgM-, and 4.2% showed PCR+, IgG+, and IgM+. In the first follow-up, after 2 weeks, from the 41 referred patients, 29 (70%) showed PCR+, IgG+, and IgM-, which confirmed the results of the first sampling. In the second follow-up, 9 (47%) patients were PCR+, IgG+, and IgM-. A correlation was observed between the first referral and the follow-ups. Also, from 71 patients, diagnosed clinically as ocular toxoplasmosis, the disease was confirmed in 73.2% and 26.8% of those suffering from other ocular infections. Of the 20 control group samples, 55% showed PCR-, IgG+, and IgM-. The sensitivity, specificity, negative and positive predictive values, and negative and positive likelihoods were analyzed for IgG and IgM antibodies and for PCR using ELISA method.<br> <strong>&nbsp;&nbsp; Conclusion: </strong>As the ophthalmologic signs of T. gondii may be mimicked by other infections, clinical methods may be complemented by laboratory approaches for a definite diagnosis. This would assist clinicians to achieve timely diagnosis and successful therapy and to control the infection.<br> &nbsp; Ocular toxoplasmosis, Toxoplasma gondii, Molecular approaches, Serology 500 504 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3329-2&slc_lang=en&sid=1 Vafa Saber Vafa.Saber125@Gmail.com 200319475328460062500 200319475328460062500 No Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran Seyyed Javad Seyyed Tabaei seyyedtabaei@sbmu.ac.ir 200319475328460062501 200319475328460062501 Yes Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Seyyed Ali Tabatabaei Dr.tabatabaei@yahoo.com 200319475328460062502 200319475328460062502 No Farabi Hospital, Tehran University of Medical Sciences, Tehran, Iran Mohammad Soleimani Solemni_md@yahoo.com 200319475328460062503 200319475328460062503 No Farabi Hospital, Tehran University of Medical Sciences, Tehran, Iran Ali Haghighi Ahaghi110@yahoo.com 200319475328460062504 200319475328460062504 No Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran