Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1397
11
1
gregorian
2019
2
1
33
1
online
1
fulltext
en
Prevalence of vaginolysin, sialidase and phospholipase genes in Gardnerella vaginalis isolates between bacterial vaginosis and healthy individuals
Microbiology and Anatomy
Microbiology and Anatomy
Original Research
Original Research
<strong> Background: </strong>Gardnerella vaginalis is considered as the predominant microorganism found in bacterial vaginosis (BV). The aim of this study was to evaluate the prevalence of virulence factors in G. vaginalis associated with BV or non-BV cases and their correlations with this disorder.<br>
<strong> Methods: </strong>A total of 102 vaginal specimens were collected from patients during their visit to Akbar Abadi hospital in Tehran, Iran. Bacterial vaginosis was determined by Nugent score and Amsel’s criteria. Polymerase chain reaction (PCR) was used for the detection of G. vaginalis 16S rRNA, vaginolysin, sialidase and phospholipase genes. To evaluate the association between the presence of vly, pho, and sld genes and BV. Pearson Chi-square test was applied using SPSS software. P-value ≤0.05 was considered as significant.<br>
<strong> Results:</strong> Totally, 27.4% of the patients were suffering from BV. Gardnerella vaginalis was found in 100% women with BV and in 56.7% women with normal vaginal discharge. The prevalence of vly, sld and pho genes in BV-associated G. vaginalis was 10 (35.7%) (95% CI [0.18, 0.53]), 19 (67.8%) (95% CI [0.51, 0.85]) and 6 (21.4%) (95% CI [0.06, 0.37]), respectively. The prevalence of the aforementioned genes in non-BV associated G. vaginalis was 20 (47.6%) (95% CI [0.33, 0.63]), 28 (66.6%) (95% CI [0.52, 0.81]), and 5 (11.9%) (95% CI [0.02, 0.22]), respectively. Our results showed no statistically significant association between the presence of the virulence genes and BV associatedness of this microorganism<strong>. </strong><br>
<strong> Conclusion: </strong>Our results showed the presence of <em>G. vaginalis </em>in all BV patients and relatively high prevalence in healthy individuals. The prevalence rates of the three virulence genes were different in BV and non-BV associated <em>G. vaginalis</em>; however, the differences were not statistically significant.
Gardnerella vaginalis, Bacterial vaginosis, PCR
517
522
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1256-1&slc_lang=en&sid=1
Rokhsareh
Mohammadzadeh
rokhsare.mohamadzade@gmail.com
200319475328460062484
200319475328460062484
No
Microbial Biotechnology Research Center & Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Behrooz
Sadeghi Kalani
behroz.sadeghi@gmail.com
200319475328460062485
200319475328460062485
No
Microbial Biotechnology Research Center & Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Maryam
Kashanian
Maryamkashanian@yahoo.com
200319475328460062486
200319475328460062486
No
Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Mojgan
Oshaghi
Mobin255@gmail.com
200319475328460062487
200319475328460062487
No
Department of Laboratory Sciences, School of Allied Health, Iran University of Medical Sciences, Tehran, Iran
Nour
Amirmozafari
amirmozafari@yahoo.com
200319475328460062488
200319475328460062488
Yes
Microbial Biotechnology Research Center & Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran