Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1398
11
1
gregorian
2020
2
1
34
1
online
1
fulltext
en
Role of inflammatory mediators (TNF-α, IL-6, CRP), biochemical and hematological parameters in type 2 diabetes mellitus patients of Kashmir, India
Endocrinology
Endocrinology
Original Research
Original Research
<strong>Background: </strong>Type II Diabetes mellitus (T2DM) is a multifactorial disease and a leading cause of premature deaths. Inflammatory cytokines are reported that they have potential to enhance insulin resistance and hence T2DM. Assessment of immunological profile in T2DM patients of Kashmir valley is unclear. So, detection of cytokines is relevant to determine the extent and direction of immune responses. The current research was taken to study the role of inflammatory mediators in T2DM along with insulin sensitivity, biochemical and hematological parameters in mountainous valley of Kashmiri population.<br>
<strong>Methods:</strong> A total of 340 subjects were selected in this study among them 160 were T2DM cases and 180 were healthy controls. Serum expression of inflammatory mediators (TNF-α and IL-6 ) were quantified by ELISA technique, WBC count was measured on Sysmax (Germany) hematology analyzer, biochemical and Immunoassay parameters were done on Abbott c4000 (USA) and Abbott C1000 (USA) fully automatic analyzer. Data was analyzed using statistical ‘software SPSS 16.1’ (Chicago, IL). For all assessments, p<0.05 were considered statistically significant.<br>
<strong>Results: </strong>The expressions of candidate cytokines (TNF-α, IL-6, CRP, and WBC) were highly significant (p<0.001) in T2DM. Among inflammatory mediators, TNF-α shows a positive correlation (p<0.001) with glycemic profile and insulin sensitivity in T2DM cases in comparison with healthy normal. Biochemical (fasting sugar, HbA1c, insulin resistance, lipid profile) and anthropometric (BMI) parameters were highly significant (p<0.001) in T2DM cases as compared to non-diabetic normal.<br>
<strong>Conclusion:</strong> Low grade inflammation and up regulation of inflammatory mediators has been purported to play a significant role in pathogenesis of T2DM. Our findings confirm that positive correlation of TNF-α and IL-6 with T2DM and insulin sensitivity. These can act as early prediction biomarkers of T2DM. Further studies on wider range of pro and anti- inflammatory cytokines i.e. mediators, in association with other biochemical, immunoassay and hematological parameters are needed to help clinicians manage and treat T2DM effectively.<br>
Inflammation, Biomarkers, Cytokines, Kashmir, Mediators, Adult onset type 2 diabetes mellitus, SMHS
33
39
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-250-5&slc_lang=en&sid=1
Haamid
Bashir
haamidb7@gmail.com
200319475328460063731
200319475328460063731
No
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Showkat Ahmad
Bhat
showkatbht@gmail.com
200319475328460063732
200319475328460063732
No
Department of Biochemistry, Govt. Medical College Doda, Kashmir, India
Sabhiya
Majid
biochemistrygmcsgr@gmail.com
200319475328460063733
200319475328460063733
Yes
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Rabia
Hamid
rabeyams@yahoo.co.in
200319475328460063734
200319475328460063734
No
Department of Biochemistry , University of Kashmir, Srinagar, India
Rakesh
Koul
rkoul7@gmail.com
200319475328460063735
200319475328460063735
No
Sheri-Maharaja Hari Singh Hospital (SMHS) & Associated Hospitals Govt. Medical College, Srinagar, Kashmir, India.
Muneeb U
Rehman
muneebjh@gmail.com
200319475328460063736
200319475328460063736
No
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Isha
Din
inshabeighh@gmail.com
200319475328460063737
200319475328460063737
No
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Javaid
Ahmad Bhat
javaidah@ymail.com
200319475328460063738
200319475328460063738
No
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Jasiya
Qadir
jasiyaqadir@gmail.com
200319475328460063739
200319475328460063739
No
Department of Biochemistry, Govt. Medical College Srinagar, Research Centre- University of Kashmir, Srinagar, India
Akbar
Masood
akbarmasood@kashmiruniversity.net
200319475328460063740
200319475328460063740
No
Department of Biochemistry , University of Kashmir, Srinagar, India