Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1383
8
1
gregorian
2004
11
1
18
3
online
1
fulltext
en
EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN BONE MARROW MESENCHYMAL STEM CELLS
Heart
Heart
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by
several investigators to enhance the repopulating potential of HUCBC. The
proliferation and expansion of human hematopoietic stem cells (HSC) in ex
vivo culture was examined with the goal of generating a suitable clinical protocol for
expanding HSC for patient transplantation. Using primary human mesenchymal
stem cells, we established a serum-free culture system to expand human primitive
progenitors and transplantable stem cells. Non-enriched cord blood CD34+ cells
were cultured on a monolayer of human mesenchymal stem cells in the presence of
tlu-ombopoietin (TPO), flt31flk2 ligand (FL), and/or stem cell factor (SCF),
interleukin 6 (IL-6), interleukin 3 (IL-3) under serum-free conditions. After I or 2
weeks of culture, cells were examined for clonogenic progenitors and percentage of
CD34+ CD38- cells. In the presence of TPO, FL, and SCF, fetal MSC cells
supported more than a 35- and 20-fold expansion of CD34+ cells and colonyforming
units in culture after 1 and 2 weeks of incubation, respectively. In addition,
LTC-IC assay were expanded more than 7- and 16-fold after 1 and 2 weeks of
culture, respectively. UCB-HSC can be expanded in culture to numbers theoretically
adequate for safe, rapid engraftment of adult patients. Additional studies are needed
to establish the functional activity of expanded UCB-HSC. This ex vivo expansion
system should prove valuable in clinical settings in which stromal cells are available
from recipients or stem cell donors.
Ex vivo expansion; Stem cells; Umbilical cord blood; Mesenchymal stem cells.
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263
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-60&slc_lang=en&sid=1
M
SOLElMANI
20031947532846003448
20031947532846003448
No
From the Hematology Dept .. School of'Medical Sciences. Tarbiat Modares University, Tehran
H
MOZDARANI
mozdaranh@modares.ac.ir
20031947532846003449
20031947532846003449
Yes
Medical Genetics Dept. School of Medical Sciences, Tarbiat Modares University, Tehran
AA
POURFATHOLLAH
20031947532846003450
20031947532846003450
No
From the Hematology Dept .. School of'Medical Sciences. Tarbiat Modares University, Tehran
Y
MORTAZAVI
20031947532846003451
20031947532846003451
No
From the Hematology Dept .. School of'Medical Sciences. Tarbiat Modares University, Tehran
K
ALIMOGHADDAM
20031947532846003452
20031947532846003452
No
Bone Marrow Transplantation Center. Shariati Hospital, Tehran,
A
HAJIFATHALI
20031947532846003453
20031947532846003453
No
the Iranian Blood Trans(ilsion Organization,Tehran
Z
ZONOBI
20031947532846003454
20031947532846003454
No
Shahid Beheshti Medical Sciences University. Tehran. Iran