Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1383
6
1
gregorian
2004
9
1
18
2
online
1
fulltext
en
IN VITRO INHIBI TI ON OF ATTACHMENT OF T HE AMERICAN TRYPANOSOME, TRYPANOSOMA RANGELI ON THE SALIVARY GLAND OF I TS VECTOR, RHODNIUS PROLIXUS, USING THE PARASITE EXTRACT AND PARTI AL PURIFICATION OF AN ATTACHMENT INHIBITOR PROTEIN
General
General
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The life cycle of the American trypanosome, Trypanosoma rangeli, in its invertebrate
host, Rhodnius prolixus, is completed by invasion into the insect salivary glands.
As a prerequisite, there is an initial recognition of the salivary gland receptors for the
adhesion/invasion by T rangeli. Therefore, the purpose of this study was to investigate
the putative receptor molecules on either the salivary gland or parasites surfaces,
which mediate the adhesion/invasion. Initially, the possible agglutination activity ofT
rangeli epimastigotes against blood cell was examined. Then, in order to investigate
the role of any receptor ligands on the surface of T rangeli in adhesion/invasion of the
parasite, GlcNAc was preferentially used to isolate a ligand protein. A 60kDa protein
bound to the GlcNAc column and was isolated from T rangeli (Tr-60) and the purity
of this protein was confirmed by reverse phase HPLC. Tr-60 also exhibited a potent
parasite inhibitory effect indicating that this protein (most probably a glycoprotein) may
act as a ligand receptor. In vivo, the development and invasion of T rangeli into the
salivary gland was not interfered with by the presence of a sugar inhibitor of the R.
prolixus hemolymph lectin
Tlypanosoma rangeli, Rhodnius prolixus, Salivary glands,P roteins, attachment.
159
166
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-96&slc_lang=en&sid=1
HR
BASSERI
hamid_basseri@hotmail.com" hamid_basseri@hotmail.com
20031947532846003586
20031947532846003586
Yes
the Department of Medical Entomology. School of Public Health, Tehran University of Medical Sciences, P0. Box 14155-6446. Tehran, Iran
NA
RATCLIFFE
20031947532846003587
20031947532846003587
No
Biomedical and Physiological Research Group, School o/Biological Sciences, University o/Wales Swansea, Singleton Park. Swansea SA2 8PP United Kingdom