en Clinical Biochemistry Clinical Biochemistry Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine Virulence of the opportunistic yeast, Candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. Cell wall mannoproteins are found to be the main cause of adherence of C. albieans to epithel ial cells in the first step of an infection process. In the present study, cell wall mannoproteins of intact yeast were purified using a simple treatment of yeast with mercaptoethanol and sodium dodecyl sulfate followed by Concanavalin A chromatography. Both electrophoretic analysis of the column effluent and Western blot analysis using polyclonal and monoclonal antibodies showed the presence of mannoproteins with molecular weight in the range of30-50 kDa. Dot blot analysis of the purified antigen with the polyclonal and monoclonal antibodies prepared in this study showed that outer membrane mannoprotein antigens were obtained successfully following the above simple purification strategy Candida albicans, Mannoproteins, Purification, Characterization 167 172 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-97&slc_lang=en&sid=1 Z FARAHNEJAD 20031947532846003588 20031947532846003588 No From the Department 0f Medical Mycology MJ RASAEE rasaee _m@modares.ac.ir. 20031947532846003589 20031947532846003589 Yes Department of Clinical Biochemistry H YADEGARI 20031947532846003590 20031947532846003590 No From the Department 0f Medical Mycology M FROUZANDEH MOGHADAM 20031947532846003591 20031947532846003591 No Department o/Medical Biotechnology, School of Medical Sciences, Tarbiat Modarres University, Tehran. I.R. Iran