Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1383
2
1
gregorian
2004
5
1
18
1
online
1
fulltext
en
COLLAGENASE ACTIVITY IN PREVOTELLA BIVIUS ISOLATED FROM PATIENTS WITH PREMATURE RUPTURE OF MEMBRANES
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Bacterial vaginosis (BV) has been considered to be the most prevalent infection
found in sexually active women. BV is thought to play an important role in the premature
rupture of membranes (PROM) and preterm birth. Preterm delivery accounts for
a substantial percentage of low birth weight infants and perinatal mortality and morbidity.
Bacteroides and Prevotella species have been isolated from the amniochorion of
women with preterm birth and PROM. Women with> 104/mL Prevotella bivius (formerly
Bacteroides bivius) have a 60-100% higher rate of preterm delivery. The purpose
of this study was to determine wether some strains of Prevotella species isolated
from PROM and BV patients produce proteases especially collagenase enzymes which
faciliate the rupture of membranes leading to preterm birth.
Vaginal specimens have been obtained from 120 women with BV and premature
rupture of membrane in 30-44 weeks gestational age. Twenty anaerobic coccobacilli
consisting of Bacteroides fragilis, black pigmented Bacteroides and Prevotella bivius
were isolated and identified. The isolates were examined for protease activity, using
porcine skine gelatin and casein as substrates by Martleys method.
Elastase and collagenase activity were detected using elastin, guinea pig skin collagen,
bovine achilles tendon col1agen, FALGPAand GP,VPKas substrates by Levenson
method.
Collagenase and elastase activity was detected in 90 and 100% of isolates.
Gelatinase and caseinase activity was detected in 40 and 50 % of isolates. Collagenase
produced by Prevotella bivia isolates was purified by ammonium sulphate precipitation,
gel filtration and ion exchange chromatography. The collagenase was cleaved from
the synthetic collagen substrate F ALGPA, and GP, VPK substrates. The activity of the
enzyme was inhibited by EDTA, Antipain and PMSF.
This study suggests that proteases produced by Prevotella bivia may be involved
in the pathogenesis of premature rupture of membrane. PROM before 37
weeks has been reported to be significantly higher among patients with Bacteroides
and Prevotella colonisation of the genital tract. The amniochorion consists of collagen
and elastin which convey physical integrity to the placenta. Collagenase and elastase
released into the genital tract may promote connective tissue destruction in the cervix
and chorioamnion membranes.
Prevotella bivius-collagenase activity- BV- PROM
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-114&slc_lang=en&sid=1
REZA
HOSSEINI DOUST
rcmb@bmsu.ac.ir
20031947532846003641
20031947532846003641
Yes
From the Research Center o/Molecular Biology, Baqiatallah University of Medical Sciences, Tehran, l.R. lran
ASHRAF
MOHABBATI MOBAREZ
20031947532846003642
20031947532846003642
No
the Department of Microbiology, Tarbiat Modarres University, Tehran, I.R. lran.