en Original Research Original Research As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI buffer, pH 8.1, resulting in recovery of 85 % IgM, 84% IgA and 33 % IgG. The comparison of our results with immunoglobulins' percentage in plasma indicates that IgM and IgA -enrichment was obtained by three times. Immunoglobulin; IgM; IgA; Enrichment; Ion exchange chromatography. 315 318 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-127&slc_lang=en&sid=1 KAMRAN MOUSAVI HOSSEINI 20031947532846003695 20031947532846003695 Yes From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran MOJGAN POURMOKHTAR 20031947532846003696 20031947532846003696 No the Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. RASSOUL DINAR VAND 20031947532846003697 20031947532846003697 No the Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. HOURI REZVAN 20031947532846003698 20031947532846003698 No From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran MOHAMMAD ALI JALILI 20031947532846003699 20031947532846003699 No From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran