Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1382
2
1
gregorian
2003
5
1
17
1
online
1
fulltext
en
ANTISENSE RNA TO THE TYPE I INSULIN-LIKE GROWTH FACTOR RECEPTOR REVERSED THE TRANSFORMED PHENOTYPE OF PC-3 HUMAN PROSTATE CANCER CELL LINE IN VITRO
Medical Genetics
Medical Genetics
Original Research
Original Research
The insulin-like growth factor I receptor (IGF-IR) plays an essential role in
the establishment and maintenance of transformed phenotype. Interference with
the IGF-IR pathway by antisense causes reversal of the transformed phenotype in
many rodent and human tumor cell lines. We stably transfected the PC-3 human
prostate cancer cell line with an IGF-IR antisense RNA expression plasmid. The
number of IGF-I receptors on the antisense-transfected PC-3 cells was reduced by
40.2% relative to the control-transfected cells. The transfected cells maintained
their high expression of IGF-IR antisense RNA for up to one year in selective
medium . The reduction in the expression of IGF-IR had no effect on the cell
growth in monolayer. The clonogenicity of antisense-transfected cells was 24.7%
of the clonogenicity of control-transfected cells in soft agar. There was a good
correlation between IGF-IR level and inhibition of transformation in soft agar.
These results indicate that reduction of IGF-IR by antisense RNA can reverse the
transformed phenotype of human prostate cancer cells.
Type I insulin-like growth factor receptor; Antisense; Transfonnation; prostate cancer; PC- 3.
59
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-182&slc_lang=en&sid=1
R
JALAL
20031947532846003908
20031947532846003908
No
A
DIZAGI
20031947532846003909
20031947532846003909
No
K
NAYERNIA
20031947532846003910
20031947532846003910
No
B
GOLIAEI
goliaei@ibb.ut.ac.ir
20031947532846003911
20031947532846003911
Yes