en Gynecology & Obstetrics Gynecology & Obstetrics Original Research Original Research <strong>Background: </strong>Endometrial cancer is the fourth most widespread cancer among females, with a growing prevalence in recent years. Management by combined therapies along with surgery, radiotherapy, and chemotherapy have improved patients&rsquo; prognoses. Besides, the development of new therapies helps preserve fertility and prognosis in aggressive tumors.<br> The purpose of this research was to identify the efficacy of metformin on the H19 long non-coding RNA expression in endometrial cancer to provide further insight into the pathogenesis and treatment of the disease.<br> &nbsp;&nbsp; <strong>Methods: </strong>A total of 23 patients with endometrial cancer, diagnosed by biopsy or diagnostic curettage, were recruited and divided into three groups, before and after metformin treatment and placebo. Real-time PCR was used to evaluate the H19 expression in cancer tissue in all patients.<br> <strong>&nbsp;&nbsp; Results:</strong> It has been observed that in endometrial tissue of the &ldquo;after-metformin&rdquo; treatment group, the H19 expression level was significantly reduced, compared with the &ldquo;before-metformin&rdquo; treatment group, but not in comparison with the placebo. These findings indicate that metformin reduced the H19 expression in endometrial cancer.<br> &nbsp;&nbsp; <strong>Conclusion:</strong> Anti-diabetic drugs, such as metformin, may be beneficial by reducing the H19 expression in endometrial cancer due to the H19 relation to cancer progression. Endometrial Cancer, Non-Coding RNA, H19 Gene, Metformin 1146 1151 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-6979-1&slc_lang=en&sid=1 Soheila Aminimoghaddam Aminimoghaddam.S@Iums.ac.ir 200319475328460090570 200319475328460090570 No Department of Obstetrics and Gynecology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Bahareh Fooladi fooladi.b@iums.ac.ir 200319475328460090571 200319475328460090571 Yes Department of Obstetrics and Gynecology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Maryam Noori Dr.Ghiasi22@Gmail.com 200319475328460090572 200319475328460090572 No Department of Obstetrics and Gynecology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Zeynab Nickhah Klashami Nickhah2016@Yahoo.com 200319475328460090573 200319475328460090573 No Metabolic Disorders Research Centre, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Iran Armita Kakavand Hamidii akakavandhamidi@gmail.com 200319475328460090574 200319475328460090574 No Metabolic Disorders Research Centre, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Iran Mahsa M. Amoli amoilmm@tums.ac.ir 200319475328460090575 200319475328460090575 No Metabolic Disorders Research Centre, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Iran