Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1380
8
1
gregorian
2001
11
1
15
3
online
1
fulltext
en
APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative
assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin
(RAHlg) were prepared. Nitrocellulose paper dotted with the antigen
was added to serially diluted sera or blood samples. The reacting antibodies were
visualized by a peroxidase system. Development of a colored insoluble substrate
was taken as a positive result. The adapted DIA was applied to test 105 serum
samples. The sensitivity and specificity and immune titer of DIA were compared
with hemagglutination inhibition (HI) test.
Rubella virus. dot immunoassay ( DIA), nitrocellulose paper. hemagglutination inhibition (HI)
171
174
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-256&slc_lang=en&sid=1
T
BAMDAD
20031947532846004154
20031947532846004154
Yes
From the Department of Virology. Facility a/Medical Sciences. Tarbiat Modarres University. Po. Box 14115-111. Tehran
MH
RODSTAI
20031947532846004155
20031947532846004155
No
From the Department of Virology. Facility a/Medical Sciences. Tarbiat Modarres University. Po. Box 14115-111. Tehran
H
SOLIMANDJAHI
20031947532846004156
20031947532846004156
No
From the Department of Virology. Facility a/Medical Sciences. Tarbiat Modarres University. Po. Box 14115-111. Tehran
M
MALEKANEH
20031947532846004157
20031947532846004157
No
the Department a/Biochemistry. Faculty of Medical Sciences. Birjand University of Medical Science's. Birdjand. I.R. Iran.