Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1380
2
1
gregorian
2001
5
1
15
1
online
1
fulltext
en
A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN
Parasitology and Mycology
Parasitology and Mycology
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Hydatid cyst fluid has been used as a source of antigen for the serodiagnosis
of Echinococcus granulosus infection. Due to cross-reactions with antigens shared
by other helminthes, the specific antigen from hydatid cyst fluid was purified by
many workers. We used a relatively simple technique for purification of specific
antigen from sheep hydatid cyst fluid. Isopycnic ultracentrifugation with 30%
KEr was used followed by SDS-PAGE to check the purity of the antigen. The
antigen was of 48 kDa and used in ELISA and IRA with high sensitivity and
specificity.
Echinococcus granulosus, specific antigen, isopycnic ultracentrifugation
49
53
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-279&slc_lang=en&sid=1
Z
ZAMANI
20031947532846004243
20031947532846004243
No
M
ASSMAR
20031947532846004244
20031947532846004244
Yes
From the Departments of Parasitology and Biochemistry, Pasteur Institute of Iran, Tehran, I.R. Iran.
N
PYAZAK
20031947532846004245
20031947532846004245
No
K
WAFAIE
20031947532846004246
20031947532846004246
No
M
ASSADIAN
20031947532846004247
20031947532846004247
No
A
AMIRKHANI
20031947532846004248
20031947532846004248
No