en Immunology Immunology Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine In vitro culture systems using bone marrow cells from BALB/C mice were set up in medium supplemented with spleen-derived medium. Bone marrow cells grown in spleen-derived medium gave rise to cultures containing >97% mast cells. The cells were used in polarisation chemotaxis assays with the intention of determining the effect of nerve tissue-derived neurotransmitters on mast cell migration. Some available neurotransmitters including (substance-p, histamine, serotonin, Dopa, noradrenaline, acetylcholine, aspartic acid, epinephrine and nerve extracts) were tested. Mast cells showed a significant morphological response to 10-3 and 10-4 M histamine, 10-9 M serotonin, and 10'2 M Dopa Nerve extracts induced some shape changes in mast cells. Mast cell, Nerve tissue mediators, Chemotaxis. 379 383 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-296&slc_lang=en&sid=1 P KERMANIZADEH 20031947532846004299 20031947532846004299 Yes From the Division of Immunology. Faculty of Medicine. Tabriz University of Medical Sciences. Tabriz. I. R. Iran P HAGAN 20031947532846004300 20031947532846004300 No . the Division of Infection & Immunity. Joseph Black Building. IBLS. University of Glasgow. Glasgow Gi2 BQQ DWT CROMPTON 20031947532846004301 20031947532846004301 No the Division of Environmental & Evolutionary Biology. T he Graham Kerr Building.IBLS. University of Glasgow. Glasgow Gi2 BQQ. UK.