Medical Journal of the Islamic Republic Of Iran

en The Prevalence and Clinical Features of Co-Infection with SARS-CoV-2 and Influenza Virus during the COVID-19 Pandemic in Semnan, Iran COVID 19 Original Research Original Research     Background: COVID-19 and influenza are both contagious respiratory diseases. Influenza virus can increase the severity of COVID-19 infection in the cold months of the year through damage to respiratory ciliated cells, which may cause an increase in hospitalization, disease symptoms and mortality rate. Therefore, the purpose of this study was to ascertain the frequency of co-infection with the influenza virus and SARS-CoV-2, as well as the impact of co-infection on clinical outcomes in hospitalized patients suffering from respiratory problems within Semnan City, Iran.    Methods: In this cross-sectional descriptive study, we investigated 1267 hospitalized patients with respiratory problems between September 2021 and March 2022. Two nasopharyngeal and oropharyngeal throat swab samples were collected from each patient and tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A, and influenza B viruses using real-time reverse-transcriptase– polymerase-chain-reaction (RT-PCR). The collected data were analyzed with χ² test, ANOVA, paired Student's t-test, and Pearson's correlation coefficient test in different groups. Analyzes were done with SPSS 26.0 software.    Results: In total, 29.6% (n = 375)  of patients had confirmed positive results for SARS-CoV-2, and their median age was 55.4 ± 24.63 years. It was found that 1.9% (n = 7) and 0.5% (n = 2) of COVID-19 patients had co-infections with influenza viruses A and B, respectively. In 2.4% of the cases, co-infection with COVID-19 and influenza was found. 8 out of 9 patients (88.8%) recovered, while one patient (11.1%) died. Co-infection did not significantly correlate with cancer (P = 0.588), diabetes (P = 0.202), hypertension (P = 0.530), or any other illness. Also, Associations of death and co-infection with diabetes, cardiovascular disease, or CKD showed that a statistically significant correlation was present only between diabetes and death. Based on the ANOVA test to look at associations of death and co-infection with diabetes, cardiovascular disease, or CKD, it showed that there was no significant association of co-infection with diabetes (P = 0.202), hypertension (P = 0.530), cancer (P = 0.588), and other diseases.    Conclusion: Although a low proportion of COVID-19 patients have influenza co-infection, the importance of such co-infection, especially in high-risk individuals and the elderly, cannot be ignored. Given the prevalence of influenza co-infection, increased coverage of flu vaccination is encouraged to mitigate the transmission of the influenza virus during the ongoing COVID-19 pandemic and reduce the risk of severe outcomes and mortality.   COVID-19, Influenza A and B, Co-infection 710 715 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-4980-2&slc_lang=en&sid=1 Mahdieh Tarahomi m.tarahomi94@gmail.com 200319475328460089529 200319475328460089529 No Semnan University of Medical Sciences, Semnan, Iran Mohammad Sadegh Shokri Koohikheyli amirshokri001997@gmail.com 200319475328460089530 009-0009-1063-6145 No Semnan University of Medical Sciences, Semnan, Iran Pegah Pooya pgahpooya1994@yahoo.com 200319475328460089531 0009-0002-2097-7639 No Department of Molecular Virology, Semnan Health Reference Laboratory, Semnan University of Medical Sciences, Semnan, Iran Majid Eslami majid.bac@gmail.com 200319475328460089532 200319475328460089532 No Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran Mahshid Vakili mahshid_vakili2011@yahoo.com 200319475328460089533 200319475328460089533 No Department of Bacteriology and Virology, Semnan University of Medical Sciences, Semnan, Iran Fatemeh Habibian parisa.h1378@gmail.com 200319475328460089534 200319475328460089534 No Semnan University of Medical Sciences, Semnan, Iran Ramtin Naderian ramtinndn@gmail.com 200319475328460089535 200319475328460089535 No Student Committee of Medical Education Development, Education Development Center, Semnan University of Medical Science, Iran Mohsen Zolfaghari Mohsen.zolfaghari.19.19@gmail.com 200319475328460089536 0009-0003-1293-7781 No Student Committee of Medical Education Development, Education Development Center, Semnan University of Medical Science, Iran Hadi Ghaffari Hghaffari@semums.ac.ir 200319475328460089537 200319475328460089537 Yes Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran