Medical Journal of the Islamic Republic Of Iran

en Effect of Herbal Bioactive Compounds on the Angiogenic Factors and Modulation of Inflammatory Mediators in the Patients with the Deep Second-Degree Burn: A Single-Blinded, Randomized Clinical Trial Burn & Reconstruction Burn & Reconstruction Original Research: Clinical Science Original Research: Clinical Science     Background: Inflammation is the first response to tissue damage. A hematoma occurs when blood leaves the damaged vessels, and platelets play an important role in this process. This study aimed to investigate the effect of herbal bioactive compounds on the angiogenic factors and modulation of inflammatory mediators in deep second-degree burn patients.    Methods: In a randomized clinical trial, 54 patients were divided into two groups :Swalin ointment (n=31) and silver sulfadiazine (SSD) (n=23). Ointments were administered every other day for 28 days. The concentration of compounds in ointment oils was measured using the GC-MS technique. Serum levels of TNF-α and IL-6 were measured on days 3, 7, and 14, and tissue levels of VEGF, FGF, and PDGF variables were measured on day 14 by ELISA method. Student t-test was used to compare the mean in 2 groups, depending on the type of normal/abnormal distribution. The chi-square test was also used to check the relationship between qualitative variables.    Results: The most common compounds in Swalin ointment were Linoleic acid (41.37%), Elaidic acid (37.45%), and Palmitic acid (9.45%), respectively. The tissue level of VEGF, FGF, and PDGF on the 14th day was higher in the Swalin group compared to the SSD group (P<0.001). The serum level of IL-6 and TNF-α in both groups increased until day 7, but gradually decreased on day 14, which was significant in the Swalin group. IL-6 serum level was significant in the Swalin group (P<0.001). The serum level of TNF-α was also significant in the Swalin group (P<0.001).    Conclusion: The present study showed that Swalin ointment, due to the presence of a wide range of fatty acids, especially linoleic acid, leads to the modulation of systemic tissue inflammation. The ingredients of the ointment, especially hemp and sesame oil, increase the tissue level of angiogenic factors and accelerate remodeling and wound healing.   Burns, Angiogenesis, Vascular Endothelial Growth Factors 116 129 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-8862-1&slc_lang=en&sid=1 Mahdi Heydari Mahdi.heydari@yahoo.com 200319475328460088432 200319475328460088432 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Seyyed Mohsen Seyyedkazemi Seyyedmohsen.seyyedkazemi@yahoo.com 200319475328460088433 0009-0004-7705-6843 No Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran Mohammad Taghi Joghataei Mohammadtaghi.joghataei@yahoo.com 200319475328460088434 200319475328460088434 No Department of Anatomy, Cellular and Molecular Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Fereshteh Mehraein Ghomi Fereshtehmehraein.ghomi@yahoo.com 200319475328460088435 200319475328460088435 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Hoorieh Mohammadi Kenari Hoorieh.mohammadi.kenari@yahoo.com 200319475328460088436 200319475328460088436 No School of Persian Medicine, Iran University of Medical Sciences, Tehran, Iran Nader Sadigh Nader.sadigh@yahoo.com 200319475328460088437 200319475328460088437 No Department of Emergency Medicine, School of Medicine, Trauma and Injury Research Center, Iran University of Medical Sciences, Tehran, Iran Auob Rustamzadeh auob2020rustamzade@gmail.com 200319475328460088438 200319475328460088438 No Department of Anatomical Sciences, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran Mehrdad Bakhtiari Mehrdad.bakhtiari@yahoo.com 200319475328460088439 200319475328460088439 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Hamidreza Alizadeh-Otaghvar alizadehotaghvarhamidreza@gmail.com 200319475328460088440 200319475328460088440 Yes Department of Plastic & Reconstructive Surgery, School of Medicine, Trauma and Injury Research Center, Shahid Motahari Burns Hospital, Iran University of Medical Sciences, Tehran, Iran