Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
8
1
gregorian
1999
11
1
13
3
online
1
fulltext
en
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
Pharmacology
Pharmacology
Original Research
Original Research
A simple, rapid and sensitive HPLC method for the determination of theophylline
(T) in human serum has been developed. An isocratic system consisting of a /-l
Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5),
and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm
at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal standard.
The serum samples deproteinated by methanol containing 8-CT and the supernatant
was injected into the HPLC system. The retention times of 5.7 and 8.1 min were
found for T and 8-CT, respectively. The linearity was checked in the range of 0.2-
30 µg/mL. Relative standard deviation for both inter-day and intra-day precision
analysis was less than 5%. No interference was observed from endogenous serum
components. Specificity was shown against some commonly co-administered drugs.
Simple and fast sample preparation, small sample volume (250 µL), precision,
reproducibility, specificity, sensitivity and high percentage recovery (98 %) make the
method to be practically useful for T monitoring in asthmatic patients.
Theophylline; Reversed-phasechromatography; 8-Chlorotheophylline; Therapeutic drug monitoring.
0
0
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-388&slc_lang=en&sid=1
H
TAJERZADEH
20031947532846004586
20031947532846004586
Yes
From the Biopharmacy Division, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR.Iran, 14155/6451.
S
SADRAY
20031947532846004587
20031947532846004587
No