Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
2
1
gregorian
1999
5
1
13
1
online
1
fulltext
en
MOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR
Drug
Drug
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fermentor. At the late exponential growth phase, crude polysaccharide wa obtained from fermentation broth by detergent-phenol extraction method and purified by ultracentrifuge differentiation technique. Analytical data reveals that by optimization of fermentation parameters, not only was the yield of production increased from 1.6 mg/L to 4.9 mg/L, but also the polysaccharide retained its native molecular stability and immunogenicity. Therefore, purified Vi-CPS can be regarded as a reliable immunogen to control typhoid fever in man.
Salmonella typhi. Vi-CPS, Polysaccharide vaccine.
37
42
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-421&slc_lang=en&sid=1
HOJJAT
AHMADI
20031947532846005815
20031947532846005815
Yes
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
BAHMAN
TABARAlE
20031947532846005816
20031947532846005816
No
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
MEHDI
NEJATI
20031947532846005817
20031947532846005817
No
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
SAEID
AL-e AGHA
20031947532846005818
20031947532846005818
No
Razi Vaccine and Serum Research Institute. Hesarak, I.R. Iran.