Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
2
1
gregorian
1999
5
1
13
1
online
1
fulltext
en
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 antigenic determinant. These vectors were transferred into the yeast. Only pDFl which contained the HBsAg gene was expressed. Some peculiar features of recombinant plasmid construction and expression of the HBsAg gene are discussed
HBV surface antigen, recombinant plasmids, Saccharomyces cerevisiae exoression vector.
67
73
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-426&slc_lang=en&sid=1
ALI A
FARHOUDI-MOGHADDAM
20031947532846005800
20031947532846005800
Yes
From the Molecular Biology Unit . Pasteur Institute. Tehran. I.R. Iran
NICKOLAY N
DOMANSKY
20031947532846005801
20031947532846005801
No
A.A
ZIAEE
20031947532846005802
20031947532846005802
No