en Biological Sciences Biological Sciences Original Research: Basic Science in Medicine Original Research: Basic Science in Medicine The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 antigenic determinant. These vectors were transferred into the yeast. Only pDFl which contained the HBsAg gene was expressed. Some peculiar features of recombinant plasmid construction and expression of the HBsAg gene are discussed HBV surface antigen, recombinant plasmids, Saccharomyces cerevisiae exoression vector. 67 73 http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-426&slc_lang=en&sid=1 ALI A FARHOUDI-MOGHADDAM 20031947532846005800 20031947532846005800 Yes From the Molecular Biology Unit . Pasteur Institute. Tehran. I.R. Iran NICKOLAY N DOMANSKY 20031947532846005801 20031947532846005801 No A.A ZIAEE 20031947532846005802 20031947532846005802 No