Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1377
11
1
gregorian
1999
2
1
12
4
online
1
fulltext
en
BRONCHODILATORY AND ANTICHOLINERGIC EFFECTS OF CARUM CARVI ON ISOLATED GUINEA PIG TRACHEAL CHAINS
Physiology
Physiology
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Carum carvi (CC) is a grassy plant which is believed to have several therapeutic
effects, including anti-asthmatic properties. We have therefore studied the
bronchodilatory and anticholinergic effects of this plant.
The bronchodilatory effects of the aqueous extract (AE), macerated extract
(ME), essential oil (EO), and 4µM theophylline (T) in comparison with saline (S)
were examined by their relaxant effects on precontracted [by 10 µM
methacholine (M)] isolated tracheal chains of guinea pigs (n=1O). The
anticholinergic effects of AE, ME, EO, and 5 nM atropine (A) were tested by
comparing the cumulative Log concentration-response curves (LCRCs) of Minduced
contraction of tracheal chains and the effective concentration of M
causing 50% of maximum response (EC50) in the presence of AE, ME, EO, and A
with that of S.
Although the bronchodilatory effects of AE, ME, and EO were lower than that
Of T (p<0.001 for all cases), they were significantly higher than the effect of S (p<
0.05 for AE, p<0.01 for ME, and p<0.005 for EO). There were also clear nonparallel
right-ward shifts in LCRCs obtained in the presence of AE, ME, and EO,
but a parallel shift in the presence of A compared to the curve obtained in the
presence of S. The slope of LCRCs obtained in the presence of AE, ME, and EO
were significantly lower than that of S (p<O.OI for AE, p<0.05 for ME, and
p<0.001 for EO). The ECso obtained in the presence of AE (p<0.005), ME
(p=O.005), EO (p<0.02), and A (p<0.001) were significantly higher than that of S.
However, the EC50 values obtained in the presence of AE, ME, EO and dose ratio
(DR-I) produced by ME were not significantly different from that of A. The
LCRCs obtained in the presence of EO on incubated tracheal chains with 1µM
propranolol and 1 µM chlorpheniramine also showed a clear non-parallel rightward
shift comparing with that of S. However, the slope of this curve was
significantly higher than that of non-incubated experiments (p<0.05).
The results of this study indicated that the bronchodilatory effect of Carum
carvi is mainly due to the non-competitive antagonistic property of this plant at
muscarinic receptors. The β stimulatory effect and/or anti-histaminic effect of EO
might be contributed to its non-competitive property. The variation between
anticholinergic behaviours of different extracts is probably due to the variation of
methods used, leading to extraction of different substances or destruction of some
substances due to high temperature.
Carum carvi, bronchodilatory, anticholinergic, guinea pig.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-437&slc_lang=en&sid=1
MH
BOSKABADY
20031947532846004728
20031947532846004728
Yes
From the Dept. of Physiology, Ghaem Medical Center, Mashhad University of Medical Sciences, Mashhad, I R. Iran.
M
TALEBI
20031947532846004729
20031947532846004729
No