Volume 2, Issue 3 (11-1988)                   Med J Islam Repub Iran 1988 | Back to browse issues page


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A. GHAVAMI NEJAD A, ZANDIEH T. INHIBITION OF14C UPTAKE BY CANDIDA ALBICANS: A NEW METHOD FOR INVESTIGATION OF INTRACELLULAR KILLING BY PHAGOCYTES. Med J Islam Repub Iran. 1988; 2 (3) :237-242
URL: http://mjiri.iums.ac.ir/article-1-1255-en.html

From the Department of Biology, Faculty of Sciences, Isfahan University of Medical Sciences, Isfahan,
Abstract:   (1778 Views)
A radio assay employing 14C-uridine to measure the intracellular killing of Candida albicans by phagocytes is presented. The principle of this new method is that after killing of Candida albicans by phagocytes RNA synthesis stops and therefore uridine uptake is diminished whilst viable candida in cultures would take up uridine and as a result, high CPM counts can be obtained within 135 min. of incubation in test cultures. Leukocytes from 30 normal subjects showed reduced C.P.M. following killing of Candida albicans.
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