Volume 7, Issue 2 (8-1993)                   Med J Islam Repub Iran 1993 | Back to browse issues page

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R ASADIKARAM G, RASAEE M, ALE AGHA S, KUMARI G, RAO P. A SPECIFIC, SENSITIVE, AND ONE-STEP EXTRACTION RADIOIMMUNOASSAY OF TESTOSTERONE FOR CLINICAL DIAGNOSIS. Med J Islam Repub Iran. 1993; 7 (2) :101-108
URL: http://mjiri.iums.ac.ir/article-1-1441-en.html
From the Department of Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, I.R.lran
Abstract:   (2231 Views)
Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3- O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure testosterone in female serum samples accurately with a high sensitivity, precision, and specificity. The coefficent of variation (CY), standard deviation (SD) and standard error of mean (SE) were all in acceptable ranges. Antibody-bound and free steroids were separated by addition of dextran coated charcoal. The method was applied to a set of clinical samples, the results of which are discussed in this communication. The assay was compared with the available imported kits using 125 I as tracer. The correlation coefficient obtained is calcualted to be r= 0.96, showing that the results obtained by these two methods are fully comparable and the assay may be replaced with the similar preparations imported from abroad.
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