Volume 19, Issue 1 (5-2005)                   Med J Islam Repub Iran 2005 | Back to browse issues page


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HASHEMI M, KARAMI TEHRANI F, GHAVAMI S, SIRATI SABET M. ADENOSINE DEAMINASE ACTIVITY IN ESTROGEN RECEPTOR POSITIVE AND NEGATIVE HUMAN BREAST CANCER CELL LINES. Med J Islam Repub Iran. 2005; 19 (1) :53-56
URL: http://mjiri.iums.ac.ir/article-1-416-en.html

Dept. of Clinical Biochemistry, Zahedan Medical University, Zahedan , Iran . , mhdhash@yahoo.com
Abstract:   (3248 Views)

 ABSTRACT

 Background: The aims of this study were to assay the activity of adenosine deaminase (ADA) in estrogen receptor positive (MCF-7) and negative (MDA-MB468) breast cancer cell lines.

 Methods: MDA-MB468 and MCF-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-LM diethylstilbestrol (DES), complete medium in the presence and absence of 111M tamoxifen for 20 hr. Adenosine deaminase activity was determined using the colorimetric method described by Guisti and Galanti.

 Results: It was found that the activity of enzyme in estrogen receptor positive (ER+) cell line (MCF-7) was significantly higher than that of estrogen receptor negative breast cancer cell line (MDA-MB468). ADA activity in MCF-7 cells cultured in the presence of tamoxifen or charcoal-striped serum was significantly lower than that of control. Furthermore addition of diethylstilbestrol (DES) to the striped serum increased the value of ADA activity to that of control. Unlike MCF-7 cells, the activity of ADA in MDA-MB468 cells remained unchanged upon treatment with tamoxifen or striped serum.

 Conclusion: These findings suggest estrogen responsiveness of ADA expression in MCF-7 cells.

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