Volume 13, Number 1 (5-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page


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AHMADI H, TABARAlE B, NEJATI M, AL-e AGHA S. MOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR. Med J Islam Repub Iran. 1999; 13 (1) :37-42
URL: http://mjiri.iums.ac.ir/article-1-970-en.html

From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
Abstract:   (2063 Views)
The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fermentor. At the late exponential growth phase, crude polysaccharide wa obtained from fermentation broth by detergent-phenol extraction method and purified by ultracentrifuge differentiation technique. Analytical data reveals that by optimization of fermentation parameters, not only was the yield of production increased from 1.6 mg/L to 4.9 mg/L, but also the polysaccharide retained its native molecular stability and immunogenicity. Therefore, purified Vi-CPS can be regarded as a reliable immunogen to control typhoid fever in man.
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