Volume 8, Issue 4 (2-1995)                   Med J Islam Repub Iran 1995 | Back to browse issues page

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ASADY KARAM G, RASAEE M. DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL. Med J Islam Repub Iran 1995; 8 (4) :243-248
URL: http://mjiri.iums.ac.ir/article-1-1364-en.html
DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL
GHR ASADY KARAM , MT RASAEE
From the Department of Biochemistry. School of Medical Sciences.
Abstract:   (3695 Views)
An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossreacted in less than 20% with 5α-dehydrotestosterone (5α DHT) . lnter- and intraassay variations and all other validation factors such as recovery, test of parallelism, etc. were well in the acceptable ranges. Comparison of testosterone values of 32 plasma specimens obtained by solid phase ELISA method and radioimmunoassay (RIA) showed a good correlation (0. 96).
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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